Table 4.
BBD severity and resistance ratings of 20 P. ostreatus strains based on screening with two methods via the inoculation of the pathogen on mushroom cap or pileus method (IMP) and on the mushroom spawned substrate method (IMSS).
| Screening with the IMP method X | Screening with the IMSS method Y | Overall disease response (DI) Z | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No | Strain ID | Origin | Type | Cap Color | Disease Severity (DS1) | Resistance Rating | Disease Severity (DS2) | Resistance Rating | Overall DI (%) | Resistance Rating | ||
| 1 | P10 | Changchun, China | C | Gray | 1.53 ± 0.39 | a* | MS | 2.63 ± 0.48 | h–i* | HS | 59.2 | HS |
| 2 | P164 | Heilongjiang, China | C | Gray | 1.57 ± 0.27 | a | MS | 1.80 ± 0.45 | d–f | S | 45.7 | S |
| 3 | P92 | Changchun, China | W | Gray | 1.77 ± 0.23 | a | MS | 1.17 ± 0.28 | c | MS | 37.1 | MS |
| 4 | P191 | Shandong, China | C | Pale gray | 2.23 ± 0.42 | b | MS | 2.20 ± 0.32 | g | HS | 59.0 | HS |
| 5 | P179 | Heilongjiang, China | C | Gray | 2.27 ± 0.26 | b | MS | 2.33 ± 0.44 | g–h | HS | 61.6 | HS |
| 6 | P297 | Yunnan, China | C | Pale gray | 2.43 ± 0.39 | b | MS | 2.23 ± 0.50 | g | HS | 61.5 | HS |
| 7 | P35 | Yunnan, China | W | Dark gray | 2.53 ± 0.48 | b–c | S | 1.60 ± 0.38 | d | S | 52.0 | S |
| 8 | P11 | Changchun, China | C | Pale gray | 2.60 ± 0.41 | b–c | S | 2.00 ± 0.42 | e–g | S | 59.3 | HS |
| 9 | P90 | Changchun, China | W | Pale gray | 2.60 ± 0.31 | b–c | S | 1.70 ± 0.37 | d–e | S | 54.3 | S |
| 10 | P67 | Changchun, China | W | Dark gray | 2.87 ± 0.28 | c–d | S | 2.96 ± 0.20 | i | HS | 78.0 | HS |
| 11 | P176 | Heilongjiang, China | C | Gray | 3.00 ± 0.39 | d | S | 0.97 ± 0.25 | b–c | MR | 46.1 | S |
| 12 | P186 | Heilongjiang, China | C | Dark gray | 3.10 ± 0.49 | d–e | S | 2.93 ± 0.44 | i | HS | 79.9 | HS |
| 13 | P279 | Yunnan, China | C | Pale gray | 3.20 ± 0.39 | d–f | S | 2.30 ± 0.37 | g–h | HS | 70.3 | HS |
| 14 | P317 | Yunnan, China | C | Pale gray | 3.20 ± 0.36 | d–g | S | 0.70 ± 0.25 | b | MR | 43.7 | S |
| 15 | P346 | Yunnan, China | C | Cream | 3.23 ± 0.42 | d–h | S | 0.00 ± 0.00 | a | HR | 32.3 | MS |
| 16 | P62 | Yunnan, China | W | Dark gray | 3.43 ± 0.35 | e–h | S | 1.83 ± 0.33 | d–f | S | 64.9 | HS |
| 17 | P173 | Heilongjiang, China | C | Dark gray | 3.57 ± 0.32 | f–i | HS | 2.63 ± 0.40 | h–i | HS | 79.6 | HS |
| 18 | P40 | Yunnan, China | C | Dark gray | 3.88 ± 0.42 | i | HS | 2.70 ± 0.48 | i | HS | 83.7 | HS |
| 19 | P0 | Jilin, China | C | Pale gray | 3.90 ± 0.32 | i | HS | 2.10 ± 0.39 | f–g | HS | 74.0 | HS |
| 20 | P167 | Heilongjiang, China | C | Dark gray | 4.33 ± 0.52 | j | HS | 1.70 ± 0.29 | d–e | S | 71.7 | HS |
X The P. ostreatus strains were cultivated in room 2 (at 18 ± 1 °C, 90% RH, 150 lux light, 500–600 ppm CO2) in 2017. Strain collection type: Wild (W) and cultivated (C).
DS1 = mean DS scores from two flushes produced/strain obtained after 48 hours of inoculation on mushroom caps/pilei of 10 replicate bags (3 caps were inoculated/flush/bag) ± standard deviation. Resistance scale based on DS1: 0–0.5 = highly resistant (HR); 0.6–1.5 = moderately resistant (MR); 1.6–2.5 = moderately susceptibly (MS); 2.6–3.5 = susceptible (S); >3.5 = highly susceptible (HS).
Y The P. ostreatus strains were cultivated in room 2 (at 18 ± 1 °C, 90% RH, 150 lux light, 500–600 ppm CO2) in 2018. DS2 = mean DS scores from two flushes produced/strain, obtained during mushroom fruiting after inoculation of P. tolaasii on the spawned substrate, calculated from 10 replicate bags (3 caps scored/flush/bag) ± standard deviation. Resistance scale based on DS2: 0-0.5 = HR; 0.6-1.0 = MR; 1.1-1.5 = MS; 1.6-2.0 = S; >2.0 = HS.
Z Overall resistance scale base on DI: 0.01–15.0% = HR; 15.1–25.0% = MR; 25.1–40.0% = MS; 40.1–55.0% = S; >55.1% = HS.
* Means having a different letter(s) in the same column differed significantly (p < 0.05) according to the DMRT.