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. 2019 Nov 17;8(4):241. doi: 10.3390/pathogens8040241

Figure 3.

Figure 3

Expression of LmxM.22.0250 in L. mexicana development, growth kinetics, and macrophage infection in vitro of the wild type (WT), Cas9, and LmDUSP1 KO cells. (A) Quantitative RT-PCR analysis of LmxM.22.0250 expression in the axenically differentiated procyclic promastigotes (pro), metacyclic promastigotes (meta), and amastigotes (ama) of the wild type (Cas9) L. mexicana. (B) Growth curves of the WT, Cas9, and LmDUSP1 KO L. mexicana. (C) The intensity of infection (number of parasites per well) was calculated for WT, LmDUSP1 KO, and Cas9-expressing L. mexicana in non-stimulated or LPS/IFN-γ stimulated primary murine macrophages. Data are summarized from three independent biological replicates. The error bars indicate standard deviation. N.S. indicates a not statistically significant difference.