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. 2019 Dec 14;161(1):bqz031. doi: 10.1210/endocr/bqz031

Figure 1.

Figure 1.

Hemorrhagic shock (HS) specifically induces the expression of Est in the liver. Female and male WT mice were subjected to sham surgery or HS/R. Serum and tissue were harvested at 24 hours after HS. (A and B) The mRNA expression of Est in the liver and lung of female (A) and male (B) mice was measured by real-time PCR. (C) The protein expression of Est in the liver and lung in female and male mice was measured by immunofluorescence staining of DAPI (blue) and Est (green). Bar is 100 μm. (D) The protein expression of Est in the liver of female and male mice was measured by western blotting. Shown to the right is the quantification of the western results. Numbers in parentheses in A and B are average real-time PCR cycle numbers. Results are presented as mean ± SEM, (n = 3 for each group). *P < 0.05; **P < 0.01, compared with the sham groups. Abbreviations: mRNA, messenger ribonucleic acid; PCR, polymerase chain reaction; R, resuscitation; SEM, standard error of the mean; WT, wild-type.