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. 2020 Jan 17;8:e8391. doi: 10.7717/peerj.8391

Isolation, sequencing, and expression analysis of 30 AP2/ERF transcription factors in apple

Huifeng Li 1,#, Qinglong Dong 2,#, Qiang Zhao 3, Song Shi 4,✉, Kun Ran 1,✉
Editor: Xiaolin Sun
PMCID: PMC6970539  PMID: 31988809

Abstract

Background

AP2/ERF transcription factors are involved in the regulation of plant growth, development, and stress responses. Our research objective was to characterize novel apple (Malus × domestica Borkh.) genes encoding AP2/ERF transcription factors involved in regulation of plant growth, development, and stress response. The transcriptional level of apple AP2/ERF genes in different tissues and under various biotic and abiotic stress was determined to provide valuable insights into the function of AP2/ERF transcription factors in apple.

Methods

Thirty full-length cDNA sequences of apple AP2/ERF genes were isolated from ‘Zihong Fuji’ apple (Malus × domestica cv. Zihong Fuji) via homologous comparison and RT-PCR confirmation, and the obtained cDNA sequences and the deduced amino acid sequences were analyzed with bioinformatics methods. Expression levels of apple AP2/ERF genes were detected in 16 different tissues using a known array. Expression patterns of apple AP2/ERF genes were detected in response to Alternaria alternata apple pathotype (AAAP) infection using RNA-seq with existing data, and the expression of apple AP2/ERF genes was analyzed under NaCl and mannitol treatments using qRT-PCR.

Results

The sequencing results produced 30 cDNAs (designated as MdERF3-8, MdERF11, MdERF16-19, MdERF22-28, MdERF31-35, MdERF39, MdAP2D60, MdAP2D62-65, and MdRAV2). Phylogenetic analysis revealed that MdERF11/16, MdERF33/35, MdERF34/39, and MdERF18/23 belonged to groups A-2, A-4, A-5, and A-6 of the DREB subfamily, respectively; MdERF31, MdERF19, MdERF4/25/28/32, MdERF24, MdERF5/6/27, and MdERF3/7/8/17/22/26 belonged to groups B-1, B-2, B-3, B-4, B-5, and B-6 of the ERF subfamily, respectively; MdAP2D60 and MdAP2D62/63/64/65 belonged to the AP2 subfamily; and MdRAV2 belonged to the RAV subfamily. Array results indicated that 30 apple AP2/ERF genes were expressed in all examined tissues to different degrees. RNA-seq results using previously reported data showed that many members of the apple ERF and DREB subfamilies were induced by Alternaria alternate apple pathotype (AAAP) infection. Under salt treatment, many members in the apple ERF and DREB subfamilies were transcriptionally up or down-regulated. Under mannitol treatment, many members of the apple ERF, DREB, and AP2 subfamilies were induced at the transcriptional level. Taken together, the results indicated that the cloned apple AP2/ERF genes were expressed in all examined tissues. These genes were up-regulated or down-regulated in response to AAAP infection and to salt or mannitol treatment, which suggested they may be involved in regulating growth, development, and stress response in apple.

Keywords: Sequencing, Expression analysis, Apple, AP2/ERF transcription factors, Abiotic stress, Biotic stress

Introduction

AP2/ERF is one of the large transcription factor families in plants that is involved in many biological processes, such as plant growth, development, and environmental stress (Chuck et al., 2002; Aharoni et al., 2004; Broun et al., 2004; Mizoi, Shinozaki & Yamaguchi-Shinozaki, 2012). Each AP2/ERF contains the AP2/ERF conserved domain that consists of 60–70 amino acid residues, which results in the name for the AP2/ERF family. The AP2 domain regulates the expression of target genes by binding to the GCC-box (Ohme-Takagi & Shinshi, 1995), the dehydration responsive element (DRE) (Sun et al., 2008; Guttikonda et al., 2014), and/or the TTG element (Wang et al., 2015). The AP2/ERF family is divided into three subfamilies (AP2, ER, and RAV) based on the similarity of amino acid sequences and number of conserved domains (Nakano et al., 2006). There are two AP2/ERF domains in the AP2 subfamily, one AP2/ERF and one B3 domain in the RAV subfamily, and one AP2/ER domain in the ERF subfamily. In addition, the ERF subfamily is divided into ER and CBF/DREB subgroups, with differences at the 14th and 19th amino acid (Sakuma et al., 2006).

AP2/ERF family members have been isolated, and their functions have been identified in many species (Xu et al., 2011; Mizoi, Shinozaki & Yamaguchi-Shinozaki, 2012; Licausi, Ohme-Takagi & Perata, 2013). Overexpression of members of the subfamily DREB in transgenic plants increased resistance to abiotic stress, such as drought (Hong & Kim, 2005; Oh et al., 2009; Fang et al., 2015), salt (Hong & Kim, 2005; Bouaziz et al., 2013), cold (Fang et al., 2015), and high temperatures (Qin et al., 2007). Also, the overexpression of ERF members not only improved the resistance to multiple biological stresses by regulating the expression of defense genes (Berrocal-Lobo, Molina & Solano, 2002; Guo et al., 2004; Dong et al., 2010; Moffat et al., 2012), but also increased resistance to abiotic stress, such as drought (Zhang et al., 2010a; Zhang et al., 2010b; Yang et al., 2016), high salt concentrations (Guo et al., 2004), freezing (Zhang & Huang, 2010), and osmotic stress (Zhang et al., 2010a). Members of the AP2 subfamily played important roles in the development of flowers, fruits, and seeds (Maes et al., 2001; Jofuku et al., 2005; Chung et al., 2010; Horstman et al., 2014). RAV members were responded to ethylene, brassinolide (BR), and biotic and abiotic stress (Mittal et al., 2014).

Apple (Malus × domestica Borkh.) is one of the most important tree fruits in the world. However, progress on the ERF transcription factors in apple is more limited than that in model plants like Arabidopsis thaliana, and most researches about apple are focused on fruit ripening and softening (Wang et al., 2007; Tacken et al., 2010; Li et al., 2016; An et al., 2017; Li et al., 2017; Han et al., 2018a; Han et al., 2018b). In this study, we obtained the AP2/ERF transcription factor in apple based on previous results (Girardi et al., 2013) and from the Plant Transcription Factor Database (http://planttfdb.cbi.pku.edu.cn/). When the 60 known transcription factors were excluded by sequence alignment in the GenBank database (Tacken et al., 2010), the other genes were cloned and analyzed. In total, 30 genes in the AP2/ERF family were obtained. Furthermore, we analyzed the phylogenetic relationships, subcellular locations, and expression levels in different tissues under different biotic and abiotic stresses for the 30 AP2/ERF genes. The results are helpful for further studying roles of AP2/ERF transcription factors played in growth, development, and biotic and abiotic stress in apple.

Materials & Methods

Plant materials

The apple cultivar ‘Gala’ (Malus × domestica cv. Gala) was used as material under stress conditions. In vitro seedlings of ‘Gala’ were cultivated on basic subculture medium (MS medium + 0.2 mg L−1 indole-3-acetic acid (IAA) + 0.8 mg L−1 6-benzylaminopurine (6-BA) + 30 g L−1 sucrose + 7 g L−1 agrose) that was changed every 30 d. The cultivation conditions were under 14-h light/10-h dark and a temperature of 24  ± 2 °C. On the 20th day on the basic subculture medium, some relatively uniform seedlings were selected and transplanted to different media. The basic subculture medium was used as the control. We added 150 mmol L−1 NaCl or 300 mmol L−1 mannitol to the basic subculture medium to create different treatments (Li et al., 2019).

Gene cloning and sequence analysis

RNA was extracted in the fully expanded leaves of ‘Zihong Fuji’ apple (Malus × domestica cv. Zihong Fuji) by the CTAB method, then cDNA was synthesized using a PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Dalian, China). Based on the nucleotide sequence of 259 identified members in the apple AP2/ERF gene family and the 60 known transcription factors in the GenBank database (Tacken et al., 2010; Velasco et al., 2010; Girardi et al., 2013), we designed primers for PCR amplification and 30 apple AP2/ERF genes were finally cloned (Table S1). The PCR reaction conditions were 94 °C for 5 min, then 35 cycles for 94 °C for 1 min 20 s, 56–60 °C for 1 min, 72 °C for 2 min, and a final extension at 72 °C for 10 min. PCR products were purified and cloned into pMD19-T vector to construct recombinant plasmids. The recombinant plasmids were transformed into the competent cells of Escherichia coli DH5α, and then the positive clones were selected.

The cDNA sequences that we obtained were used as queries in BLASTN searches against NCBI (https://www.ncbi.nlm.nih.gov/). The open reading frame (ORF) and amino acid sequences were analyzed by DNAMAN 6.0 software. The phylogenetic tree was constructed by MEGA 6 software according to the unrooted Neighbour Joining (NJ) method with execution parameters: the Poisson correction, pairwise deletion, and bootstrap (1,000 replicates), using full-length amino acid sequences from AP2/ERF proteins of apple and Arabidopsis. The conserved domains were predicted by Pfam 26.0 (http://pfam.xfam.org/) and the Conserved Domains program in NCBI (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). CELLO v.2.5 (http://cello.life.nctu.edu.tw/), PSORT (https://psort.hgc.jp/form.html), and SoftBerry ProtComp 9.0 (http://linux1.softberry.com/) were used to predict subcellular locations (Dong et al., 2018a; Dong et al., 2018b; Dong et al., 2018c; Hao & Qiao, 2018).

Subcellular localization analysis

The full-length cDNA without the stop codon of MdERF28 was introduced into the pCAMBIA2300-GFP vector. The fusion vectors were then introduced into Agrobacterium tumefaciens strain EHA105 and then infiltrated into tobacco leaves. Those infected tissues were analyzed 72 h after infiltration, under a fluorescence microscope (BX63; Olmypus, Tokyo, Japan).

Gene expression analysis

The expression data for the AP2/ERF gene family in different tissues were obtained at Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) with GEO accession number GSE42873 (Celton et al., 2014). These existing data included a set of expression arrays from 16 different apple tissues (from 10 different genotypes of apple: leaf_M14 (fully developed), fruit_M20_100 DAM (100 days after anthesis)/_harvest (harvested at maturity), leaf_M49 (fully developed), flower_M67, flower / fruit _M74_100 DAM/_Harvest, root (growing root tip)/ stem (fully developed)/ seedling (10 days old)_GD, seedling (10 days old)_X4102, root (growing root tip)/ stem (fully developed)_X8877, seed (dormant seed)_X4442 × X2596 and seed (dormant seed)_X3069 × X922), with two biological replicates for each tissue, and a known array probe was used as the MDP identification number in apple genome database V1.0. The RNA-seq data for AP2/ERF response to AAAP was from Zhu et al. (2017).

The RNA was extracted from the treated tissues of ‘Gala’ using a RNeasy Plant Mini Kit (QIAGEN, China, Item No. 74903), and the cDNA was synthesized using the PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Dalian, China). The qRT-PCR primers (Table S1) were designed based on the 3′- or 5′-UTR of AP2/ERF genes, and then qRT-PCR was conducted using a 3-step method by BIO-RAD IQ5 (USA) with MdMDH RNA as the internal reference gene (Perini et al., 2014). Three independent biological replicates were used for calculations. Each 20 µL qRT-PCR reaction mixture consisted of SYBR Green Master I 10 µL, 5 µmol L−1 forward prime 1 µL, 5 µmol L−1 reverse prime 1 µL, template 1 µL, and ddH2O 7 µL. qRT-PCR conditions were 95 °C for 3 min, then 40 cycles for 95 °C for 10 s, 58.5 °C for 30 s, 72 °C for 15 sand, after annealing to 55 °C, the temperature was increased 0.5 °C every 7 s till 95 °C, with 81 cycles in total. The 2−ΔΔCT method was used to analyze the data (Livak & Schmittgen, 2001).

Results

Cloned genes in the AP2/ERF family in apple

Based on the nucleotide sequence of 259 identified members in the apple AP2/ERF gene family and the 60 known transcription factors in the GenBank database (Tacken et al., 2010; Velasco et al., 2010; Girardi et al., 2013), the other primers for PCR amplification were designed, and a total of 30 genes in the apple AP2/ERF family were cloned (Table 1). Homology alignment for the amino acid information showed that all the MdAP2/ERF proteins contained an AP2 conserved domain (Fig. 1). Both MdAP2D60 and MdAP2D62-65 had two AP2 conserved domains, and MdRAV2 had one B3 conserved domain (Fig. 1).

Table 1. The AP2/ERF genes in apple.

Gene name V1.0 gene IDa GDDH13 gene IDb GeneBank accession GDDH13 Chromosome location ORF Amino acid MW PI Group
MdERF3 MDP0000119204 MD14G1226300 MG099812 Chr14:30769541-30771506 1029 342 38.731 4.763 B6
MdERF4 MDP0000322279 MD04G1228800 MG099813 Chr04:30908814-30909560 747 248 27.933 4.795 B3
MdERF5 MDP0000464704 MD11G1052100 MG099814 Chr11:4448529-4449446 918 305 33.478 6.05 B5
MdERF6 MDP0000190504 MD03G1049900 MG099815 Chr03:3981184-3982008 825 274 30.132 7.242 B5
MdERF7 MDP0000290880 MD15G1172600 MG099816 Chr15:13418043-13419309 708 235 26.092 9.006 B6
MdERF8 MDP0000759299 MD16G1043500 MG099817 Chr16:3058483-3060008 1032 343 38.168 4.514 B6
MdERF11 MDP0000290585 MD17G1089700 MG099820 Chr17:7361577-7363808 594 197 21.478 5.857 A2
MdERF16 MDP0000153866 MD04G1165400 MG099825 Chr04:25593675-25596164 1476 491 55.192 4.949 A2
MdERF17 MDP0000127123 MD06G1125700 MG099826 Chr06:26773748-26774617 870 289 31.312 5.64 B6
MdERF18 MDP0000246184 MD04G1009000 MG099827 Chr04:1044443-1045735 1293 430 47.099 9.09 A6
MdERF19 MDP0000308922 MD17G1152400 MG099828 Chr17:14092036-14095154 1164 387 42.728 4.698 B2
MdERF22 MDP0000287350 MD15G1124900 MG099831 Chr15:9070265-9071680 642 213 23.903 6.365 B6
MdERF23 MDP0000764803 MD17G1244300 MG099832 Chr17:29279809-29281131 1338 445 49.63 7.101 A6
MdERF24 MDP0000190237 MD14G1147100 MG099833 Chr14:23978106-23980121 630 209 23.033 9.918 B4
MdERF25 MDP0000689946 MD10G1286300 MG099834 Chr10:37571420-37572034 615 204 22.67 9.731 B3
MdERF26 MDP0000279733 MD17G1220600 MG099835 Chr17:26952408-26953440 429 142 15.885 5.821 B6
MdERF27 MDP0000854039 MD01G1214500 MG099836 Chr12:30790168-30791244 750 249 27.666 3.996 B5
MdERF28 MDP0000805422 MD05G1306900 MG099837 Chr05:43876234-43876827 573 190 20.896 9.165 B3
MdERF31 MDP0000457509 MD10G1191300 MG099840 Chr10:28815601-28816149 549 182 20.062 10.012 B1
MdERF32 MDP0000235313 MD16G1216900 MG099841 Chr16:21318271-21318960 555 184 20.958 6.433 B3
MdERF33 MDP0000652413 MD02G1060200 MG099842 Chr02:4815948-4816775 828 275 29.869 4.844 A4
MdERF34 MDP0000125673 MG099843 477 158 17.134 8.467 A5
MdERF35 MDP0000228713 MD07G1099500 MG099844 Chr07:10964414-10968649 1203 400 44.015 7.624 A4
MdERF39 MDP0000122739 MD15G1396500 MG099848 Chr15:49614193-49614798 606 201 22.232 5.245 A5
MdAP2D60 MDP0000187703 MD15G1064600 MG099849 Chr15:4496397-4499611 1956 651 71.47 7.209 AP2
MdAP2D62 MDP0000121984 MD13G1252700 MG099851 Chr13:27049619-27053293 1668 555 60.599 8.049 AP2
MdAP2D63 MDP0000314518 MD12G1075200 MG099852 Chr12:9108198-9111657 1407 468 50.657 8.133 AP2
MdAP2D64 MDP0000281079 MD01G1113400 MG099853 Chr01:22726643-22730520 1275 424 46.804 8.297 AP2
MdAP2D65 MDP0000801540 MD02G1190000 MG099854 Chr02:17483494-17487257 1962 653 72.224 7.145 AP2
MdRAV2 MDP0000939633 MD16G1047700 MG099860 Chr16:3329564-3330772 1206 401 43.821 9.241 RAV
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Notes.

a

V1.0 gene ID represents gene ID from apple V1.0 database (Velasco et al., 2010).

b

GDDH13 gene ID represents gene ID from apple GDDH13 v1.1 database.

Figure 1. Sequence analysis of the AP2 and B3 domain in apple AP2/ERF proteins.

Figure 1

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The AP2 and B3 domains were reconstructed based on the alignment of the apple conserved AP2 and B3 regions. Sequence alignment was generated by DNAMAN 6.0 software. Sequence logo was built by online software WebLogo 3.0. The heights of symbols within each stack indicate the relative frequency of each amino acid at that position.

Phylogenetic analysis of AP2/ERF proteins in apple

The MdAP2/ERF proteins were clustered and analyzed using MEGA6 software, and the known MdAP2/ERF protein types in Arabidopsis thaliana were used to identify the type of apple AP2/ERF protein. There were four subfamilies, DREB, ERF, RAV, and AP2 in the apple AP2/ERF protein family; DREB included groups A-1, A-2, A-3, A-4, A-5, and A-6, and ERF contained groups B-1, B-2, B-3, B-4, B-5 and B-6. Further, proteins MdERF11/16, MdERF33/35, MdERF34/3, and MdERF18/23 were clustered into groups A-2, A-4, A-5, and A-6 in the DREB subfamily, respectively. MdERF31, MdERF19, MdERF4/25/28/32, MdERF24, MdERF5/6/27, and MdERF3/7/8/17/22/26 were clustered into groups B1, B-2, B-3, B-4, B-5, and B-6 in the ERF subfamily, respectively. Proteins MdAP2D60 and MdAP2D62-MdAP2D65 were clustered into the AP2 subfamily; MdRAV2 was clustered into the RAV subfamily (Fig. 2, Table 1).

Figure 2. Phylogenetic relationships and subfamily classification of AP2/ERF proteins from apple and Arabidopsis.

Figure 2

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Unrooted Neighbour Joining (NJ) phylogenetic tree was constructed with MEGA 6 software using full-length amino acid sequences from AP2/ERF proteins of apple and Arabidopsis. The tree was classified into four subfamilys (DREB: A1–A6, ERF: B1–B6, AP2 and RAV).

Subcellular locations of AP2/ERF proteins in apple

Subcellular localization of AP2/ERF proteins was performed by SoftBerry ProtComp 9.0, CELLO, and PORST using their protein sequences. All prediction results indicated that MdERF3-8, MdERF11, MdERF16-19, MdERF22-28, MdERF33-35, MdERF39, MdAP2D60, MdAP2D62-65, and MdRAV2 were target to nuclear (Table 2). To further verification of these subcellular locations revealed by the online software, the MdERF28-GFP fusion protein was performed to detect the subcellular location of MdERF28 protein and a transient transfection assay into tobacco leaves. The GFP control was ubiquitously distributed throughout the cell, whereas MdERF28-GFP fusion protein was predominantly detected in the nucleus (Fig. 3), indicating that MdERF28 was localized in the nucleus.

Table 2. The information in predicting apple AP2/ERF subcellular localization.

Location Nuclear Plasma membrane Extracellular Cytoplasmic Mitochondrial Endoplasm. retic Peroxisomal Golgi Chloroplast Vacuolar
MdERF3 4.41 1.16 0 2.19 1.25 0.25 0.53 0 0 0.2
MdERF4 9.99 0 0 0 0 0 0 0 0.01 0
MdERF5 9.99 0.01 0 0 0 0 0 0 0 0
MdERF6 9.99 0 0 0 0 0 0 0 0.01 0
MdERF7 9.99 0 0 0 0 0 0 0 0.01 0
MdERF8 6.04 0.34 0.27 0.35 2.43 0.16 0 0 0.41 0
MdERF11 10 0 0 0 0 0 0 0 0 0
MdERF16 4.9 0.53 0.18 2.43 1.34 0.28 0.26 0 0 0.08
MdERF17 5.61 0.36 1.04 1.08 1.44 0 0.33 0.04 0.1 0
MdERF18 9.97 0 0 0 0 0 0 0 0.03 0
MdERF19 9.98 0 0 0 0 0 0 0 0.02 0
MdERF22 9.96 0.04 0 0 0 0 0 0 0 0
MdERF23 9.46 0 0.03 0.3 0.2 0 0 0 0 0.01
MdERF24 9.98 0.02 0 0 0 0 0 0 0 0
MdERF25 9.99 0 0 0 0 0 0 0 0 0
MdERF26 9.99 0.01 0 0 0 0 0 0 0 0
MdERF27 4.35 0.98 0.64 0.16 3.3 0.1 0.22 0 0.23 0.02
MdERF28 10 0 0 0 0 0 0 0 0 0
MdERF31 9.9 0 0 0 0 0 0 0 0.09 0.01
MdERF32 9.97 0 0 0 0 0 0 0 0.03 0
MdERF33 9.99 0 0 0 0 0 0 0 0.01 0
MdERF34 9.99 0 0 0 0 0 0 0 0 0
MdERF35 5.69 1.01 0.43 1.1 1.09 0 0 0 0.69 0
MdERF39 9.99 0 0 0 0 0 0 0 0 0
MdAP2D60 10 0 0 0 0 0 0 0 0 0
MdAP2D62 10 0 0 0 0 0 0 0 0 0
MdAP2D63 9.99 0 0.01 0 0 0 0 0 0 0
MdAP2D64 9.98 0 0 0 0 0 0 0 0.02 0
MdAP2D65 10 0 0 0 0 0 0 0 0 0
MdRAV2 9.99 0 0.01 0 0 0 0 0 0 0
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Figure 3. Subcellular localization assay of the MdERF28 protein.

Figure 3

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(A) Fluorescence microscopy image of GFP; (B) bright-field image of GFP; (C) GFP merged image; (D) fluorescence microscopy image of MdERF28-GFP; (E) Bright-field image of MdERF28-GFP; (F) MdERF28-GFP merged image. Scale bar = 50 mm.

Expression analysis of 30 AP2/ERF gene family in apple

The array (GSE42873) in 16 different apple tissues in GEO (https://www.ncbi.nlm.nih.gov/geo/) was used to evaluate the expression level of the AP2/ERF gene family in different tissues (Fig. 4). The 30 AP2/ERF genes exhibited diverse expression patterns among the various tissues (Fig. 4).

Figure 4. Expression profiles of apple AP2/ERF genes in various tissues.

Figure 4

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The data of apple AP2/ERF expression (GSE42873) in 16 different were searched at GEO database in NCBI. The heat map of apple AP2/ERF genes was generated by TIGR MeV v4.8.1 software.

Further, we detected the expression level of the response of the AP2/ERF gene family to AAAP infection using RNA-seq with existing data (>two-fold and FDR<0.001) (Zhu et al., 2017). MdERF16 in A2, MdERF35 in A4, MdERF23 in A6, MdERF25/28/32 in B3, MdERF6/27 in B5, and MdERF8 in B6 were all up-regulated in the response of apples’ AAAP infection (Fig. 5 and File S3). Particularly, the expression level of B3 in MdERF32 was increased significantly, which was 12.6-folds by 18 h post inoculation (HPI). Expression levels of MdERF23 in A6, MdERF25 in B3, MdERF28 in B3, and MdERF27 in B5 were all increased, which were 18.2, 8.4, 16.2, and 8.7-fold by 72 HPI, respectively. During the early (12 HPI) and intermediate (18 and 36 HPI) phase of infection, expression levels of MdERF4 in B3 and MdERF5 in B5 were increased at the beginning and then decreased later, and expression of MdERF4 was 4.6-fold by 18 HPI. Expression levels of MdERF22 in B6 and MdAP2D65 was down-regulated on 72 HPI (Fig. 5 and File S3). The relative expression level of other genes did not change significantly (Fig. 5 and File S3).

Figure 5. Expression profiles of apple AP2/ERF genes in response to Alternaria alternata apple pathotype infection.

Figure 5

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(A) Expression profiles of 21 apple AP2/ERF genes in response to AAAP infection; (B) expression profiles of five apple AP2/ERF genes in response to AAAP infection; (C) expression profiles of three apple AP2/ERF genes in response to AAAP infection. The expression data of apple AP2/ERF genes in response to AAAP infection were obtained from supplementary data previously published study (Zhu et al., 2017). The heat map of apple AP2/ERF genes was generated by TIGR MeV v4.8.1 software.

The AP2/ERF gene family expression in ‘Gala’ seedlings under mannitol and NaCl stress was analyzed by qRT-PCR. Under NaCl stress, eight members in the AP2/ERF family were up-regulated, which included MdERF16 in A2, MdERF23 in A6, MdERF25/28/32 in B3, MdERF24 in B4, MdERF17 in B6, and MdRAV2 (Fig. 6). Among them, the expression level of MdERF23, MdERF25, and MdERF28 were increased more than 10 times when treated for 48 h compared with that of the control. MdERF11 in A2, MdERF33 in A4, MdERF34 in A5, MdERF18 in A6, MdERF31 in B1, MdERF4 in B3, MdERF5 in B5, and MdERF22/26 in B6 were down-regulated. Expression levels of MdERF5 and MdERF39 were only 0.04 and 0.23 times that of the control, respectively, when treated with NaCl for 24 h, but expression level of MdERF39 was increased to 3.11 times that of the control when treated for 48 h. The other AP2/ERF genes under NaCl stress had almost the same expression level compared with that of the control (Fig. 6).

Figure 6. Expression heatmap of apple AP2/ERF genes under normal growth, mannitol and salt treatments.

Figure 6

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The expression data of apple AP2/ERF genes under normal growth, mannitol and salt treatments were obtained from qRT-PCR. The heat map of apple AP2/ERF genes was generated by TIGR MeV v4.8.1 software.

Under mannitol treatment condition, the relative expression levels of MdERF11 (A2), MdERF33 (A4), MdERF39 (A5), MdERF31 (B1), MdERF19 (B2), MdERF28/32 (B3), MdERF5/6/27 (B5), MdERF7 (B6), and MdAP2D60/62/64/65 were increased compared with the control, MdERF39 (A5) reached 5.96 times that of the control at 24 h, and MdERF11 (A2) and MdAP2D65 reached 7.99 and 10.7 times that of the control, respectively, when treated for 48 h (Fig. 6).The relative expression level of MdERF25 (B3) and MdRAV2 were inhibited compared with that of the control, but the relative expression level of other AP2/ERF genes did not change significantly under mannitol treatment (Fig. 6).

Discussion

Based on the draft genome sequence of the domesticated apple (Malus × domestica) and the highly conserved domain in the AP2/ERF transcription factors of plants, 259 genes in the apple AP2/ERF family were selected for analyzing ERF transcription factors in apple genome database ver 1.0 (Velasco et al., 2010; Girardi et al., 2013). In this study, we cloned 30 apple AP2/ERF genes, which belonged to the AP2, ERF, DREB, and RAV subfamilies of AP2/ERF, and their changes in expression level in different tissues were analyzed under AAAP infection, and NaCl and mannitol stresses.

ERF is one the largest transcription factor families in plants. The A. thaliana genome contained 147 AP2/ERF proteins, which were divided into the AP2, ERF (ERF and DREB), and RAV sub-families based on their similarity in amino acid sequences and domain number (Nakano et al., 2006; Mizoi, Shinozaki & Yamaguchi-Shinozaki, 2012). The 30 genes cloned in this study were divided into four subfamilies; 8, 16, 5, and 1 gene belonged to the subfamilies DREB, ERF, AP2, and RAV, respectively (Fig. 2). AAAP infection, NaCl stress, and mannitol stress all affected the expression of MdERF4/25/28/32 in the B3 group at transcriptional level, except for MdERF4 under mannitol stress. In A. thaliana, AtERF1, AtERF2, AtERF5, and AtERF6 in the B3 group, which could be induced by osmotic stress (Moffat et al., 2012), were responded to Saprophytic bacteria by up-regulating the downstream resistance genes PDF1.2 and b-CHI, resulting in enhanced resistance to S. bacteria infection (Fujimoto et al., 2000; Berrocal-Lobo, Molina & Solano, 2002; Lorenzo et al., 2003; Moffat et al., 2012). Alfafa exhibited increased resistance from MtERF1-1 in the B3 group that up-regulated the resistance downstream gene PDF1.2 (Anderson et al., 2010). In wheat, TaPIEP1 in the B3 group was up-regulated by Bipolaris sorokiniana, which boosting disease resistance (Dong et al., 2010). Transgenic tobacco plants had enhanced resistance to Tobacco Mosaic Virus and brown spot through overexpression of NtERF5 and GbERF2 (Fischer & Droge-Laser, 2004; Zuo et al., 2007). The transgenic A. thaliana with SpERF1, which was the ERF member of the B3 group in Stipa purpurea, had increased drought tolerance when SpERF1 was up-regulated (Yang et al., 2016). In this study, MdERF4/25/28/32 was clustered into the B3 group of ERF and was up-regulated significantly under AAAP infection and NaCl stress; also, mannitol stress had some effects on MdERF4/25/28/32 expression (Figs. 5 and 6). These results indicated that MdERF4/25/28/32 may play important roles in response to various biotic and abiotic stress.

Several studies have proved that the DREB transcription factor subfamily was important for abiotic stress (Nakano et al., 2006). For example, A. thaliana showed increased tolerance to high-salt and drought by overexpression of certain DREB transcription factors that included DREB2A and DREB2B in the A2 group, HARDY in the A4 group, and RAP2.4 in the A6 group. DREB2C, DREB2D, and DREB2F in A. thaliana played an important role in high-salt stress (Nakano et al., 2006; Sakuma et al., 2006; Karaba et al., 2007; Qin et al., 2007; Lin, Park & Wang, 2008). Drought tolerance in maize was enhanced by DREB2A overexpression in the A2 group (Qin et al., 2007). Overexpression of the PsAP2 gene in the A6 group of Papaver somniferum enhanced the resistance of transgenic tobacco to pathogenic bacteria, salt, and mannitol stresses (Mishra et al., 2015). In this study, under mannitol stress, MdERF11 in the A2 group, MdERF33 in the A4 group, and MdERF39 in the A5 group were up-regulated at transcriptional level. Seven genes were induced by NaCl at transcriptional level. Three of them, MdERF16 in A4, MdERF39 in A5, and MdERF23 in A6, were up-regulated at transcriptional level under NaCl stress, and four genes, which included MdERF11 in A2, MdERF33 in A4, MdERF34 in A5, and MdERF18 in A6, were down-regulated. In addition, there were four genes, which included MdERF16 in A2, MdERF35 in A4, and MdERF23 in A6, were up-regulated at transcriptional level by AAAP infection (Figs. 5 and 6). These results showed that the DREB transcription factors cloned in this study might be important for responding to abiotic stress, and some members might play a role in response to biotic stress.

The AP2 subfamily may be important for plant growth and development (Maes et al., 2001; Jofuku et al., 2005; Chung et al., 2010; Horstman et al., 2014), but also be critical for defending against biotic and abiotic stress (Park et al., 2001; Yi et al., 2004). For example, the overexpression of the Tsi1 gene improved tobacco’s tolerance to pathogenic bacteria and osmotic stress (Park et al., 2001), and the CaPF1 gene in Capsicum annuum cv. Bukang responded to ethylene (ET), jasmonic acid (JA), and cold stress, and its overexpression improved A. thaliana resistance to low temperature and to infection by Pseudomonas syringae pv. tomato DC3000 (Yi et al., 2004). In this study, MdAP2D65 in AP2 responded to AAAP infection only at transcriptional level, but it did not respond to NaCl stress, and MdAP2D60/62/64/65 were up-regulated by mannitol stress (Figs. 5 and 6). These results indicated that MdAP2D60/62/64/65 had some effect on osmotic stress, and MdAP2D65 might be involved in responding to biotic stress.

Conclusions

Thirty novel AP2/ERF genes have been successfully isolated from Malus domestica, which belong to DREB, ERF, AP2, and RAV subfamily. Results of a known array and RNA-seq analysis using existing data as well as qRT-PCR-based transcription profiling indicated that 30 apple AP2/ERF genes were expressed in all examined tissues at different expression levels, and responded differentially to various stresses, suggesting that these genes may be involved in the regulation of growth, development, and stress responses in apple. These results serve as the theoretical basis for understanding the biological function and regulation of AP2/ERF transcription factors in apple.

Supplemental Information

File S1. cDNA sequences of 30 apple AP2/ERF TFs.
Click here for additional data file. (29.4KB, docx)
DOI: 10.7717/peerj.8391/supp-1
File S2. The expression data of apple AP2/ERF genes under normal growth, mannitol and salt treatments.
Click here for additional data file. (13.4KB, xlsx)
DOI: 10.7717/peerj.8391/supp-2
File S3. The expression level of the response of apple AP2/ERF genes to AAAP infection.
Click here for additional data file. (39.5KB, xlsx)
DOI: 10.7717/peerj.8391/supp-3
Table S1. Application of primers and sequences.
Click here for additional data file. (17.8KB, docx)
DOI: 10.7717/peerj.8391/supp-4

Acknowledgments

The authors would like to thank Thomas Alan Gavin, Professor Emeritus, Cornell University; Xu Yi, Cornell University; and Professor Ma Fangfang, Shandong Agricultural University, for help in revising our English composition.

Funding Statement

This work was supported by Shandong Agricultural Good Cultivar Project (Grant No. 2016LZGC034), the National Natural Science Foundation of China (Grant No. 31501742), the Natural Science Foundation of Shandong Province (Grant No. ZR2019MC071) and the Agricultural Science and Technology Innovation Project of SAAS (CXGC2016A03 and CXGC2018F03). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Contributor Information

Song Shi, Email: shisong@njau.edu.cn.

Kun Ran, Email: rkrl001@126.com.

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Huifeng Li conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Qinglong Dong performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Qiang Zhao performed the experiments, prepared figures and/or tables, and approved the final draft.

Song Shi conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Kun Ran conceived and designed the experiments, performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The MdERF3-8 sequences are available at GenBank: MG099812, MG099813, MG099814, MG099815, MG099816 and MG099817.

The MdERF11 sequence is available at GenBank: MG099820.

The MdERF16-19 sequences are available at GenBank: MG099825, MG099826, MG099827 and MG099828.

The MdERF22-28 sequences are available at GenBank: MG099831, MG099832, MG099833, MG099834, MG099835, MG099836 and MG099837.

The MdERF31-35 sequences are available at GenBank: MG099840, MG099841, MG099842, MG099843 and MG099844.

The MdERF39 sequence is available at GenBank: MG099848.

The MdAP2D60 sequence is available at GenBank: MG099849.

The MdAP2D62-65 sequences are available at GenBank: MG099851, MG099852, MG099853 and MG099854.

The MdRAV2 sequence is available at GenBank: MG099860.

Data Availability

The following information was supplied regarding data availability:

The expression level of apple AP2/ERF genes in different tissues is available at NCBI GEO: GSE42873.

The expression level of the response of apple AP2/ERF genes to AAAP infection is available at https://www.frontiersin.org/articles/10.3389/fpls.2017.00022/full and in the Supplemental File.

References

  • Aharoni et al. (2004).Aharoni A, Dixit S, Jetter R, Thoenes E, Van Arkel G, Pereira A. The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis. The Plant Cell. 2004;16(9):2463–2480. doi: 10.1105/tpc.104.022897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • An et al. (2017).An J, Wang X, Yao J, Ren Y, You C, Wang X, Hao Y. Apple MdMYC2 reduces aluminum stress tolerance by directly regulating MdERF3 gene. Plant and Soil. 2017;418(1–2):255–266. doi: 10.1007/s11104-017-3297-7. [DOI] [Google Scholar]
  • Anderson et al. (2010).Anderson JP, Lichtenzveig J, Gleason C, Oliver RP, Singh KB. The B-3 ethylene response factor MtERF1-1 mediates resistance to a subset of root pathogens in Medicago truncatula without adversely affecting symbiosis with Rhizobia. Plant Physiology. 2010;154(2):861–873. doi: 10.1104/pp.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Berrocal-Lobo, Molina & Solano (2002).Berrocal-Lobo M, Molina A, Solano R. Constitutive expression of Ethylene-Response-Factor1 in Arabidopsis confers resistance to several necrotrophic fungi. The Plant Journal. 2002;29(1):23–32. doi: 10.1046/j.1365-313x.2002.01191.x. [DOI] [PubMed] [Google Scholar]
  • Bouaziz et al. (2013).Bouaziz D, Pirrello J, Charfeddine M, Hammami A, Jbir R, Dhieb A, Bouzayen M, Gargouri-Bouzid R. Overexpression of StDREB1 transcription factor increases tolerance to salt in transgenic potato plants. Molecular Biotechnology. 2013;54(3):803–817. doi: 10.1007/s12033-012-9628-2. [DOI] [PubMed] [Google Scholar]
  • Broun et al. (2004).Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL. WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(13):4706–4711. doi: 10.1073/pnas.0305574101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Celton et al. (2014).Celton JM, Gaillard S, Bruneau M, Pelletier S, Aubourg S, Martin-Magniette ML, Navarro L, Laurens F, Renou JP. Widespread anti-sense transcription in apple is correlated with siRNA production and indicates a large potential for transcriptional and/or post-transcriptional control. New Phytologist. 2014;203(1):287–299. doi: 10.1111/nph.12787. [DOI] [PubMed] [Google Scholar]
  • Chuck et al. (2002).Chuck G, Muszynski M, Kellogg E, Hake S, Schmidt RJ. The control of spikelet meristem identity by the branched silkless1 gene in maize. Science. 2002;298(5596):1238–1241. doi: 10.1126/science.1076920. [DOI] [PubMed] [Google Scholar]
  • Chung et al. (2010).Chung MY, Vrebalov J, Alba R, Lee J, McQuinn R, Chung JD, Klein P, Giovannoni J. A tomato (Solanum lycopersicum) APETALA2/ERF gene, SlAP2a, is a negative regulator of fruit ripening. The Plant Journal. 2010;64(6):936–947. doi: 10.1111/j.1365-313X.2010.04384.x. [DOI] [PubMed] [Google Scholar]
  • Dong et al. (2018a).Dong Q, Duan D, Zhao S, Xu B, Luo J, Wang Q, Huang D, Liu C, Li C, Gong X, Mao K, Ma F. Genome-wide analysis and cloning of the apple stress-associated protein gene family reveals MdSAP15, which confers tolerance to drought and osmotic stresses in transgenic Arabidopsis. International Journal of Molecular Sciences. 2018a;19(9) doi: 10.3390/ijms19092478. Article 2478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Dong et al. (2010).Dong N, Liu X, Lu Y, Du L, Xu H, Liu H, Xin Z, Zhang Z. Overexpression of TaPIEP1, a pathogen-induced ERF gene of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana. Functional and Integrative Genomics. 2010;10(2):215–226. doi: 10.1007/s10142-009-0157-4. [DOI] [PubMed] [Google Scholar]
  • Dong et al. (2018b).Dong Q, Mao K, Duan D, Zhao S, Wang Y, Wang Q, Huang D, Li C, Liu C, Gong X, Ma F. Genome-wide analyses of genes encoding FK506-binding proteins reveal their involvement in abiotic stress responses in apple. BMC Genomics. 2018b;19(1) doi: 10.1186/s12864-018-5097-8. Article 707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Dong et al. (2018c).Dong Q, Zhao S, Duan D, Tian Y, Wang Y, Mao K, Zhou Z, Ma F. Structural and functional analyses of genes encoding VQ proteins in apple. Plant Science. 2018c;272:208–219. doi: 10.1016/j.plantsci.2018.04.029. [DOI] [PubMed] [Google Scholar]
  • Fang et al. (2015).Fang Z, Zhang X, Gao J, Wang P, Xu X, Liu Z, Shen S, Feng B. A buckwheat (Fagopyrum esculentum) DRE-Binding transcription factor gene, FeDREB1, enhances freezing and drought tolerance of transgenic Arabidopsis. Plant Molecular Biology Reporter. 2015;33(5):1510–1525. doi: 10.1007/s11105-015-0851-4. [DOI] [Google Scholar]
  • Fischer & Droge-Laser (2004).Fischer U, Droge-Laser W. Overexpression of NtERF5, a new member of the tobacco ethylene response transcription factor family enhances resistance to tobacco mosaic virus. Molecular Plant-Microbe Interactions. 2004;17(10):1162–1171. doi: 10.1094/MPMI.2004.17.10.1162. [DOI] [PubMed] [Google Scholar]
  • Fujimoto et al. (2000).Fujimoto SY, Ohta M, Uusi A, Shinshi H, Ohme-Takagi M. Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. The Plant Cell. 2000;12(3):393–404. doi: 10.1105/tpc.12.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Girardi et al. (2013).Girardi CL, Rombaldi CV, Cero JD, Nobile PM, Laurens F, Bouzayen M, Quecini V. Genome-wide analysis of the AP2/ERF, superfamily in apple and transcriptional evidence of ERF involvement in scab pathogenesis. Scientia Horticulturae. 2013;151:112–121. doi: 10.1016/j.scienta.2012.12.017. [DOI] [Google Scholar]
  • Guo et al. (2004).Guo ZJ, Chen XJ, Wu XL, Ling JQ, Xu P. Overexpression of the AP2/EREBP transcription factor OPBP1 enhances disease resistance and salt tolerance in tobacco. Plant Molecular Biology. 2004;55(4):607–618. doi: 10.1007/s11103-004-1521-3. [DOI] [PubMed] [Google Scholar]
  • Guttikonda et al. (2014).Guttikonda SK, Valliyodan B, Neelakandan AK, Tran LS, Kumar R, Quach TN, Voothuluru P, Gutierrez-Gonzalez JJ, Aldrich DL, Pallardy SG, Sharp RE, Ho TH, Nguyen HT. Overexpression of AtDREB1D transcription factor improves drought tolerance in soybean. Molecular Biology Reports. 2014;41(12):7995–8008. doi: 10.1007/s11033-014-3695-3. [DOI] [PubMed] [Google Scholar]
  • Han et al. (2018b).Han Z, Hu Y, Lv Y, Rose JKC, Sun Y, Shen F, Wang Y, Zhang X, Xu X, Wu T, Han Z. Natural variation underlies differences in ETHYLENE RESPONSE FACTOR17 activity in fruit peel degreening. Plant Physiology. 2018b;176(3):2292–2304. doi: 10.1104/pp.17.01320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Han et al. (2018a).Han S, Nan Y, Qu W, He Y, Ban Q, Lv Y, Rao J. Exogenous γ-aminobutyric acid treatment that contributes to regulation of malate metabolism and ethylene synthesis in apple fruit during storage. Journal of Agricultural and Food Chemistry. 2018a;66(51):13473–13482. doi: 10.1021/acs.jafc.8b04674. [DOI] [PubMed] [Google Scholar]
  • Hao & Qiao (2018).Hao L, Qiao X. Genome-wide identification and analysis of the CNGC gene family in maize. PeerJ. 2018;6:e5816. doi: 10.7717/peerj.5816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Hong & Kim (2005).Hong JP, Kim WT. Isolation and functional characterization of the CaDREBLP1 gene encoding a dehydration-responsive element binding-factor like protein 1 in hot pepper (Capsicum annuum L. cv. Pukang) Planta. 2005;220(6):875–888. doi: 10.1007/s00425-004-1412-5. [DOI] [PubMed] [Google Scholar]
  • Horstman et al. (2014).Horstman A, Willemsen V, Boutilier K, Heidstra R. AINTEGUMENTA-LIKE proteins: hubs in a plethora of networks. Trends in Plant Science. 2014;19(3):146–157. doi: 10.1016/j.tplants.2013.10.010. [DOI] [PubMed] [Google Scholar]
  • Jofuku et al. (2005).Jofuku KD, Omidyar PK, Gee Z, Okamuro JK. Control of seed mass and seed yield by the floral homeotic gene APETALA2. Proceedings of the National Academy of Sciences of the United States of America. 2005;102(8):3117–3122. doi: 10.1073/pnas.0409893102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Karaba et al. (2007).Karaba A, Dixit S, Greco R, Aharoni A, Trijatmiko KR, Marsch-Martinez N, Krishnan A, Nataraja KN, Udayakumar M, Pereira A. Improvement of water use efficiency in rice by expression of HARDY, an Arabidopsis drought and salt tolerance gene. Proceedings of the National Academy of Sciences of the United States of America. 2007;104(39):15270–15275. doi: 10.1073/pnas.0707294104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Li et al. (2019).Li H, Dong Q, Zhao Q, Ran K. Genome-wide identification, expression profiling, and protein-protein interaction properties of ovate family proteins in apple. Tree Genetics and Genomes. 2019;15 doi: 10.1007/s11295-019-1354-5. Article 45. [DOI] [PubMed] [Google Scholar]
  • Li et al. (2016).Li T, Jiang Z, Zhang L, Jiang Z, Tan D, Wei Y, Yuan H, Li T, Wang A. Apple (Malus domestica) MdERF2 negatively affects ethylene biosynthesis during fruit ripening by suppressing MdACS1 transcription. The Plant Journal. 2016;88(5):735–748. doi: 10.1111/tpj.13289. [DOI] [PubMed] [Google Scholar]
  • Li et al. (2017).Li T, Xu Y, Zhang L, Ji Y, Tan D, Yuan H, Wang A. The jasmonate-activated transcription factor MdMYC2 regulates ETHYLENE RESPONSE FACTOR and ethylene biosynthetic genes to promote ethylene biosynthesis during apple fruit ripening. The Plant Cell. 2017;29(6):1316–1334. doi: 10.1105/tpc.17.00349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Licausi, Ohme-Takagi & Perata (2013).Licausi F, Ohme-Takagi M, Perata P. APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors: mediators of stress responses and developmental programs. New Phytologist. 2013;199(3):639–649. doi: 10.1038/s41598-018-29526-z. [DOI] [PubMed] [Google Scholar]
  • Lin, Park & Wang (2008).Lin RC, Park HJ, Wang HY. Role of Arabidopsis RAP2.4 in regulating light- and ethylene-mediated developmental processes and drought stress tolerance. Molecular Plant. 2008;1(1):42–57. doi: 10.1093/mp/ssm004. [DOI] [PubMed] [Google Scholar]
  • Livak & Schmittgen (2001).Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 2001;25(4):402–408. doi: 10.1006/meth.2001.1262. [DOI] [PubMed] [Google Scholar]
  • Lorenzo et al. (2003).Lorenzo O, Piqueras R, Sanchez-Serrano JJ, Solano R. Ethylene response factor1 integrates signals from ethylene and jasmonate pathways in plant defense. The Plant Cell. 2003;15(1):165–178. doi: 10.1105/tpc.007468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Maes et al. (2001).Maes T, Van de Steene N, Zethof J, Karimi M, D’Hauw M, Mares G, Van Montagu M, Gerats T. Petunia AP2-like genes and their role in flower and seed development. The Plant Cell. 2001;13(2):229–244. doi: 10.1105/tpc.13.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Mishra et al. (2015).Mishra S, Phukan UJ, Tripathi V, Singh DK, Luqman S, Shukla RK. PsAP2 an AP2/ERF family transcription factor from Papaver somniferum enhances abiotic and biotic stress tolerance in transgenic tobacco. Plant Molecular Biology. 2015;89(1–2):173–186. doi: 10.1007/s11103-015-0361-7. [DOI] [PubMed] [Google Scholar]
  • Mittal et al. (2014).Mittal A, Gampala SS, Ritchie GL, Payton P, Burke JJ, Rock CD. Related to ABA-Insensitive3(ABI3)/Viviparous1 and AtABI5 transcription factor coexpression in cotton enhances drought stress adaptation. Plant Biotechnology Journal. 2014;12(5):578–589. doi: 10.1111/pbi.12162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Mizoi, Shinozaki & Yamaguchi-Shinozaki (2012).Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K. AP2/ERF family transcription factors in plant abiotic stress responses. Biochimica et Biophysica Acta. 2012;1819(2):86–96. doi: 10.1016/j.bbagrm.2011.08.004. [DOI] [PubMed] [Google Scholar]
  • Moffat et al. (2012).Moffat CS, Ingle RA, Wathugala DL, Saunders NJ, Knight H, Knight MR. ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis. PLOS ONE. 2012;7(4):e35995. doi: 10.1371/journal.pone.0035995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Nakano et al. (2006).Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiology. 2006;140(2):411–432. doi: 10.1104/pp.105.073783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Oh et al. (2009).Oh SJ, Kim YS, Kwon CW, Park HK, Jeong JS, Kim JK. Overexpression of the transcription factor AP37 in rice improves grain yield under drought conditions. Plant Physiology. 2009;150(3):1368–1379. doi: 10.1104/pp.109.137554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Ohme-Takagi & Shinshi (1995).Ohme-Takagi M, Shinshi H. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. The Plant Cell. 1995;7(2):173–182. doi: 10.1105/tpc.7.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Park et al. (2001).Park JM, Park CJ, Lee SB, Ham BK, Shin R, Paek KH. Overexpression of the tobacco Tsi1 gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco. The Plant Cell. 2001;13(5):1035–1046. doi: 10.1105/tpc.13.5.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Perini et al. (2014).Perini P, Pasquali G, Margis-Pinheiro M, De Oliviera PRD, Revers LF. Reference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus×domestica Borkh.) Molecular Breeding. 2014;34(3):829–842. doi: 10.1007/s11032-014-0078-3. [DOI] [Google Scholar]
  • Qin et al. (2007).Qin F, Kakimoto M, Sakuma Y, Maruyama K, Osakabe Y, Tran LS, Shinozaki K, Yamaquchi-Shinozaki K. Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L. The Plant Journal. 2007;50(1):54–69. doi: 10.1111/j.1365-313X.2007.03034.x. [DOI] [PubMed] [Google Scholar]
  • Sakuma et al. (2006).Sakuma Y, Maruyama K, Osakabe Y, Qin F, Seki M, Shinozaki K, Yamaguchi Shinozaki K. Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. The Plant Cell. 2006;18(5):1292–1309. doi: 10.1105/tpc.105.035881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Sun et al. (2008).Sun S, Yu JP, Chen F, Zhao TJ, Fang XH, Li YQ, Sui SF. TINY, a dehydration-responsive element (DRE)-binding protein-like transcription factor connecting the DRE- and ethylene-responsive element-mediated signaling pathways in Arabidopsis. Journal of Biological Chemistry. 2008;283(10):6261–6271. doi: 10.1074/jbc.M706800200. [DOI] [PubMed] [Google Scholar]
  • Tacken et al. (2010).Tacken E, Ireland H, Gunaseelan K, Karunairetnam S, Wang D, Schultz K, Bowen J, Atkinson RG, Johnston JW, Putterill J. The role of ethylene and cold temperature in the regulation of the apple POLYGALACTURONASE1 gene and fruit softening. Plant Physiology. 2010;153(1):294–305. doi: 10.1104/pp.109.151092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Velasco et al. (2010).Velasco R, Zharkikh A, Affourtit J, Dhingra A, Cestaro A, Kalyanaraman A, Fontana P, Bhatnagar SK, Troggio M, Pruss D, Salvi S, Pindo M, Baldi P, Castelletti S, Cavaiuolo M, Coppola G, Costa F, Cova V, Dal Ri A, Goremykin V, Komjanc M, Longhi S, MagnArgonaute P, Malacarne G, Malnoy M, Micheletti D, Moretto M, Perazzolli M, Si-Ammour A, Vezzulli S, Zini E, Eldredge G, Fitzgerald LM, Gutin N, Lanchbury J, Macalma T, Mitchell JT, Reid J, Wardell B, Kodira C, Chen Z, Desany B, Niazi F, Palmer M, Koepke T, Jiwan D, Schaeffer S, Krishnan V, Wu C, Chu VT, King ST, Vick J, Tao Q, Mraz A, Stormo A, Stormo K, Bogden R, Ederle D, Stella A, Vecchietti A, Kater MM, Masiero S, Lasserre P, Lespinasse Y, Allan AC, Bus V, Chagne D, Crowhurst RN, Gleave AP, Lavezzo E, Fawcett JA, Proost S, Rouze P, Sterck L, Toppo S, Lazzari B, Hellens RP, Durel CE, Gutin A, Bumgarner RE, Gardiner SE, Skolnick M, Egholm M, Van de Peer Y, Salamini F, Viola R. The genome of the domesticated apple (Malus×domestica Borkh.) Nature Genetics. 2010;42(10):833–839. doi: 10.1038/ng.654. [DOI] [PubMed] [Google Scholar]
  • Wang et al. (2007).Wang A, Tan D, Takahashi A, Li TZ, Takeo H. MdERFs, two ethylene-response factors involved in apple fruit ripening. Journal of Experimental Botany. 2007;58(13):3743–3748. doi: 10.1093/jxb/erm224. [DOI] [PubMed] [Google Scholar]
  • Wang et al. (2015).Wang L, Wang C, Qin L, Liu W, Wang Y. ThERF1 regulates its target genes via binding to a novel cis-acting element in response to salt stress. Journal of Integrative Plant Biology. 2015;57(10):838–847. doi: 10.1111/jipb.12335. [DOI] [PubMed] [Google Scholar]
  • Xu et al. (2011).Xu ZS, Chen M, Li LC, Ma YZ. Functions and application of the AP2/ERF transcription factor family in crop improvement. Journal of Integrative Plant Biology. 2011;53(7):570–585. doi: 10.1111/j.1744-7909.2011.01062.x. [DOI] [PubMed] [Google Scholar]
  • Yang et al. (2016).Yang Y, Dong C, Li X, Du J, Qian M, Sun X, Yang Y. A novel AP2/ERF transcription factor from Stipa purpurea leads to enhanced drought tolerance in Arabidopsis thaliana. Plant Cell Reports. 2016;35(11):2227–2239. doi: 10.1007/s00299-016-2030-y. [DOI] [PubMed] [Google Scholar]
  • Yi et al. (2004).Yi SY, Kim JH, Joung YH, Lee S, Kim WT, Yu SH, Choi D. The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis. Plant Physiology. 2004;136(1):2862–2874. doi: 10.1104/pp.104.042903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Zhang & Huang (2010).Zhang Z, Huang R. Enhanced tolerance to freezing in tobacco and tomato overexpressing transcription factor TERF2/LeERF2 is modulated by ethylene biosynthesis. Plant Molecular Biology. 2010;73(3):241–249. doi: 10.1007/s11103-010-9609-4. [DOI] [PubMed] [Google Scholar]
  • Zhang et al. (2010b).Zhang Z, Li F, Li D, Zhang H, Huang R. Expression of ethylene response factor JERF1 in rice improves tolerance to drought. Planta. 2010b;232(2):765–774. doi: 10.1007/s00425-010-1208-8. [DOI] [PubMed] [Google Scholar]
  • Zhang et al. (2010a).Zhang H, Liu W, Wan L, Li F, Dai L, Li D, Zhang Z, Huang R. Functional analyses of ethylene response factor JERF3 with the aim of improving tolerance to drought and osmotic stress in transgenic rice. Transgenic Research. 2010a;19(5):809–818. doi: 10.1007/s11248-009-9357-x. [DOI] [PubMed] [Google Scholar]
  • Zhu et al. (2017).Zhu L, Ni W, Liu S, Cai B, Xing H, Wang S. Transcriptomics analysis of apple leaves in response to Alternaria alternate apple pathotype infection. Frontiers in Plant Science. 2017;8 doi: 10.3389/fpls.2017.00022. Article 22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • Zuo et al. (2007).Zuo KJ, Qin J, Zhao JY, Ling H, Zhang LD, Cao YF, Tang KX. Over-expression GbERF2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes. Gene. 2007;391(1–2):80–90. doi: 10.1016/j.gene.2006.12.019. [DOI] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

File S1. cDNA sequences of 30 apple AP2/ERF TFs.
Click here for additional data file. (29.4KB, docx)
DOI: 10.7717/peerj.8391/supp-1
File S2. The expression data of apple AP2/ERF genes under normal growth, mannitol and salt treatments.
Click here for additional data file. (13.4KB, xlsx)
DOI: 10.7717/peerj.8391/supp-2
File S3. The expression level of the response of apple AP2/ERF genes to AAAP infection.
Click here for additional data file. (39.5KB, xlsx)
DOI: 10.7717/peerj.8391/supp-3
Table S1. Application of primers and sequences.
Click here for additional data file. (17.8KB, docx)
DOI: 10.7717/peerj.8391/supp-4

Data Availability Statement

The following information was supplied regarding data availability:

The expression level of apple AP2/ERF genes in different tissues is available at NCBI GEO: GSE42873.

The expression level of the response of apple AP2/ERF genes to AAAP infection is available at https://www.frontiersin.org/articles/10.3389/fpls.2017.00022/full and in the Supplemental File.

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