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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Shock. 2020 Jun;53(6):737–743. doi: 10.1097/SHK.0000000000001420

Figure 2. Hydrogen sulfide gas is decreased in normoxic conditions with ablation of conventional H2S producing enzymes.

Figure 2.

A) Representative PCR blots demonstrating effective decrease in mRNA levels with siRNA transfection. Stem cells were incubated with negative control (Scramble) siRNA, as well as siRNA to CBS, MPST, and CTH. B) Analysis of H2S production demonstrates a decrease under normoxic conditions when CTH and MPST siRNAs were used. *=p<0.05 vs. Scramble (Total N=40/group. Experiments were repeated three times and averaged:16+16+8).