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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Shock. 2020 Jun;53(6):737–743. doi: 10.1097/SHK.0000000000001420

Figure 3. Hydrogen sulfide gas is not decreased in hypoxic conditions with ablation of conventional H2S producing enzymes.

Figure 3.

A) Representative PCR blots demonstrating effective decrease in mRNA levels with siRNA transfection. Stem cells were incubated with negative control (Scramble) siRNA, as well as siRNA to CBS, MPST, and CTH. B) Analysis of H2S production demonstrates no change in H2S in siRNA knockdown groups compared to Scramble during hypoxia. (Total N=40/group. Experiments were repeated three times and averaged: 16+16+8).