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. 2019 Sep 16;257(1):299–317. doi: 10.1007/s00709-019-01438-3

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 8 — Histochemical and fluorescence tests of glandular (a–e, h–l, m–o) and non-glandular trichomes (g, l) in O. picridis. a, b Fluorescence of essential oil with Neutral Red fluochrome in the TRITC (a) and Cy5 (b) filter sets. c–g Autofluorescence of essential oil in the Cy5 (c, d, f, g) and TRITC (e) filter sets. f Cells of sepal epidermis. h Staining of sesquiterpenes with conc. H₂SO₄. i Fluorescence of terpenes contain steroids with antimony trichloride in the Cy5 filter set. j Staining of acidic polysaccharides with Ruthenium Red. k, l Staining of neutral polysaccharides with Periodic acid-Schiff’s reagent (PAS reaction). m–o Staining of alkaloids with Wagner reagent. Scale bars = 100 μm (b, f), 50 μm (d, i, l), 30 μm (a, c, e, g, h, j, k, m–o)