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. 2019 Apr 7;16(1):70–85. doi: 10.1080/15548627.2019.1598750

Figure 4.

Figure 4.

CA7-4 directly bonded with MIR877-3P and MIR5680. (a–d) qPCR analysis of MIR877-3P and MIR5680 in VECs after overexpression or knockdown CA7-4 for 48 h. (e–h) VECs were treated with NG, HG, HG+3BDO, or 3BDO (NG) for 24 h, qPCR analysis of MIR877-3P and MIR5680. (i,j) Luc-CA7-WT, Luc-CA7-4-MIR877-3P-Mut, and Luc-CA7-4- MIR5680-Mut plasmids were separately co-transfected into HEK293T cells with NC, MIR877-3P mimics or MIR5680 mimics for 24 h. Detecting luciferase activity. (k–m) RNA binding protein immunoprecipitation experiments were performed by using AGO2 antibody in VECs after treatment with NG, 3BDO (30 μM), HG and HG+3BDO (30 μM) for 24 h. IgG was used as a negative control. The fold enrichment of pulled-down CA7-4 was detected by qPCR analysis. (*, p < 0.05; **, p < 0.01; n = 3.).