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. 2018 Sep 13;14(12):2139–2154. doi: 10.1080/15548627.2018.1509607

Figure 8.

Figure 8.

Fluorescence in situ hybridization reveals the majority of Mapt/Tau mRNA in the cell body with a minority in distal dendrites. (a-d) Mapt/Tau mRNA in rat hippocampal neurons (DIV 21–25) cultured in MFCs monitored by FISH with a rat Mapt/Tau mRNA probe. Axons and dendrites were visualized by immunostaining with pan-MAPT antibody K9JA (blue, b) and anti-MAP2 antibody (red, a) respectively. Images of the dendrites on the neuritic side are shown. Note that Mapt/Tau mRNA (green puncta, c) can be found in distal dendrites. Arrows indicate a sparse distribution of Mapt/Tau mRNA in distal dendrites. Scale bar: 5 µm. (e) Quantification of the average number of Mapt/Tau mRNA puncta in the cell body (~ 170) vs a distal dendrite (~ 2). Error bars, SEM from n = 45 cell bodies and 106 distal dendrites from 3 individual experiments.