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. 2020 Jan 1;10(4):1649–1677. doi: 10.7150/thno.40919

Figure 2.

Figure 2

NGF enhances neuronal regrowth following PNI. (A) A representative micrograph of NF-200 (green) and MBP (red) immunofluorescence in each group. DAPI: nuclear staining (blue). (B, C) Quantification of NF-200 and MBP-positive areas per 100 µm2 in each group. Data are presented as the mean ± SEM; n = 4 rats per group. MBP: F(2, 9) = 29.77, ***Psham vs PNI < 0.001, *PPNI vs PNI+NGF = 0.019; NF-200: F(2, 9) = 31.01, ***Psham vs PNI < 0.001, *PPNI vs PNI+NGF = 0.018. (D) RT-PCR analysis of the expression of myelinated and functional response genes in the lesion nerve treated with/without NGF at 14 days post-injury. Data are presented as the mean ± SEM. n = 3 independent experiments. MBP F(2, 6) = 49.51, ***Psham vs PNI < 0.001, **PPNI vs PNI+NGF = 0.0091; MPZ F(2, 6) = 76.08, ***Psham vs PNI < 0.001, **PPNI vs PNI+NGF = 0.0062; MAG F(2, 6) = 33.03, ***Psham vs PNI < 0.001, *PPNI vs PNI+NGF = 0.048; GAP43 F(2, 6) = 69.15, **Psham vs PNI = 0.0043, ***PPNI vs PNI+NGF < 0.001; MAP-2: F(2, 6) = 46.07, ***Psham vs PNI < 0.001, *PPNI vs PNI+NGF = 0.024.