Table 2. Normalized spectral count of proteins detected by mass spectrometry.
Name | Molecular Weight in kDa (MW) |
Average quantitative Value (AQV) |
Rough Stoichiometric Peptide Abundance (RSPA)* |
T. thermophila
Homologs** |
Human homologs*** | Localization in C. reinhardtii |
---|---|---|---|---|---|---|
TUA1 | 50 | 1077.97 | 215.59 | TBA_TETTH | TUBA1C | Doublet |
TUB1 | 50 | 625.46 | 125.09 | TBB_TETTH | TUBB4B | Doublet |
RIB72 | 72 | 116.72 | 16.21 | TTHERM_00143690 | EFHC1 | MIP |
PACRG | 25 | 38.39 | 15.35 | TTHERM_00446290 | PACRG | IJ |
PF16 | 50 | 74.09 | 14.82 | TTHERM_000157929 | SPAG6 | Central Pair |
RSP9 | 30 | 41.79 | 13.93 | TTHERM_00430020 | RSPH9 | Radial Spoke |
FAP86 | 30 | 36.11 | 12.04 | - | - | Doublet |
FAP1 | 22 | 26.46 | 12.03 | - | - | Doublet |
FAP52 | 66 | 79.12 | 11.99 | TTHERM_01094880 | CFAP52 | MIP |
FAP20 | 22 | 26.08 | 11.86 | TTHERM_00418580 | CFAP20 | IJ |
FAP126 | 15 | 16.89 | 11.26 | - | CFAP126 | MIP |
RSP1 | 88 | 98.73 | 11.22 | TTHERM_00047490 | RSPH1 | Radial Spoke |
FAP115 | 27 | 29.98 | 11.10 | TTHERM_00193760 | - | Doublet |
FAP106 | 27 | 29.81 | 11.04 | TTHERM_00137550 | ENKUR | IJ? |
Tektin | 53 | 57.61 | 10.87 | - | TEKT5 | IJ? |
RSP3 | 57 | 60.55 | 10.62 | TTHERM_00566810 | RSPH3 | Radial Spoke |
FAP252 | 39 | 39.97 | 10.25 | TTHERM_00899430 | CETN3 | Axonemal |
RSP2 | 77 | 77.95 | 10.12 | - | CALM2 | Radial Spoke |
FAP161 | 43 | 43.50 | 10.12 | TTHERM_00155380 | CFAP161 | Axonemal |
IDA4 | 29 | 27.29 | 9.41 | TTHERM_00841210 | DNALI1 | Dynein |
FAP107 | 26 | 23.66 | 9.10 | - | FLG2 | Axonemal |
DHC2 | 457 | 414.12 | 9.06 | TTHERM_01027670 | DNAH1 | Dynein |
FAP12 | 54 | 48.85 | 9.05 | - | DAGLB | Cytoplasmic |
RSP7 | 34 | 30.62 | 9.01 | TTHERM_00194419 | CALML5 | Radial Spoke |
RSP5 | 56 | 49.32 | 8.81 | - | - | Radial Spoke |
FAP230 | 45 | 39.59 | 8.80 | - | - | Axonemal |
FAP77 | 29 | 23.88 | 8.24 | TTHERM_00974270 | CFAP77 | Axonemal |
FAP55 | 111 | 90.47 | 8.15 | - | MYH14 | Axonemal |
FAP90 | 28 | 22.35 | 7.98 | - | WBP11 | Axonemal |
RSP10 | 24 | 19.10 | 7.96 | TTHERM_00378600 | RSPH1 | Radial Spoke |
FAP71 | 32 | 24.86 | 7.77 | TTHERM_00077710 | EWSR1 | Axonemal |
EEF1 | 51 | 39.04 | 7.66 | TTHERM_00655820 | Multiple | Axonemal |
FAP182 | 49 | 36.69 | 7.49 | TTHERM_01049330 | C9orf116 | Axonemal |
Rib43a | 43 | 32.06 | 7.46 | TTHERM_00624660 TTHERM_00641119 |
RIBC2 | MIP |
FAP45 | 59 | 43.20 | 7.32 | TTHERM_001164064 | CFAP45 | MIP |
*RSPA was calculated by (AQV)/(MW)*10.
** T. thermophila homologs were BLAST searched using the Uniprot database.
***Human homologs were taken from the ChlamyFP project (Pazour et al., 2005).