Figure 3. Experimental validation of stress responsive AS events by reverse transcription RT-PCR in tea leaves.

Five AS genes (under CK, DT, HT and HD) were analyzed using RT-PCR to validate the RNA-Seq results. The RT-PCR validation confirmed the computational prediction of AS genes including (A) CsMYB59, (B) CsHSP90-5 (heat shock protein 90-5), (C) CsCLASRP (CLK4-associating serine/arginine rich protein), (D) CsCDPK1 (Calcium-dependent protein kinases) and (E) CsNPR1 (nonexpressor of pathogenesis-related genes).