Table 4.

Recoveries of tylosin-spiked and tilmicosin-spiked testing by icELISA and HPLC-MS/MS (n = 3) ^a.

			icELISA			HPLC-MS/MS
Samples	Spiked	Spiked level (ng/mL)	Measured (ng/mL) (mean ± SD b)	Recoveries (%)	CV (%)	Measured (ng/mL) (mean ± SD)	Recoveries (%)	CV (%)
Milk	Tylosin	25	21.6 ± 1.6	86.4	7.4	20.16 ± 0.41	80.6	2.0
		50	41.8 ± 6.0	83.5	14.4	34.15 ± 2.67	68.3	7.8
		100	88.4 ± 5.4	88.4	6.1	78.61 ± 0.71	78.6	0.9
	Tilmicosin	25	24.7 ± 2.2	98.7	8.9	28.67 ± 0.17	114.7	0.6
		50	54.8 ± 2.0	109.5	3.6	55.05 ± 0.71	110.1	1.3
		100	83.0 ± 3.5	83.0	4.2	114.35 ± 2.66	114.4	2.3
Drinking water	Tylosin	5	4.6 ± 0.2	92.2	4.3	3.61 ± 0.41	72.1	11.3
		10	7.6 ± 0.2	76.4	2.6	6.78 ± 0.91	67.8	13.4
		20	19.8 ± 0.6	99.2	3.0	14.52 ± 1.18	72.6	8.2
	Tilmicosin	5	4.4 ± 0.6	87.0	13.6	3.40 ± 0.27	67.9	7.9
		10	9.1 ± 0.1	91.1	1.1	6.65 ± 0.03	66.5	0.5
		20	17.2 ± 1.4	85.9	8.1	14.11 ± 0.81	70.6	5.7
Tap water	Tylosin	5	4.1 ± 0.1	81.9	2.4	5.44 ± 0.01	108.7	0.1
		10	8.3 ± 0.3	82.8	3.6	11.04 ± 0.72	110.4	6.6
		20	17.7 ± 0.7	88.2	4.0	22.85 ± 0.18	114.3	0.8
	Tilmicosin	5	4.2 ± 0.3	84.5	7.1	3.21 ± 0.14	64.3	4.3
		10	8.0 ± 1.1	80.0	13.8	6.65 ± 0.21	66.5	3.2
		20	18.8 ± 2.1	94.0	11.2	14.66 ± 0.45	73.3	3.1
Environmental water	Tylosin	5	3.9 ± 0.1	77.2	2.6	4.29 ± 0.13	85.9	3.0
		10	8.8 ± 0.3	87.9	3.4	9.57 ± 0.68	95.7	7.1
		20	17.0 ± 0.2	85.1	1.2	18.37 ± 1.36	91.8	7.4
	Tilmicosin	5	5.1 ± 0.1	101.7	2.0	3.24 ± 0.13	64.9	4.1
		10	7.9 ± 0.1	79.2	1.3	8.10 ± 0.30	81.0	3.7
		20	18.1 ± 1.2	90.3	6.6	15.20 ± 1.08	76.0	7.1

^a The spiked positive samples were tested per concentration by icELISA and HPLC-MS/MS. ^b Standard deviation.