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. 2019 Dec 12;9(12):864. doi: 10.3390/biom9120864

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Size distribution plots of multiple mutants of AZ_95–228-ODC heterodimers. (A) AZ_95–228-ODC, (B) AZ_95–228-3Mα1-ODC, (C) AZ_95–228-3Mα1_D154A-ODC, (D) AZ_95–228-3Mα1_E161A-ODC, (E) AZ_95–228-5Mα1-ODC, (F) AZ_95–228-3Mα1_E142A-ODC, (G) AZ_95–228-3Mα1_H171A-ODC, (H) AZ_95–228-3Mα1_K178A-ODC, (I) AZ_95–228_E142A/H171A/K178A-ODC, and (J) AZ_95–228-8M-ODC. The sedimentation velocity data in each figure were globally fitted with the SEDPHAT program to acquire K_d values for the AZ_95–228-ODC heterodimers shown in Table 2.