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. 2020 Jan 20;9:e53473. doi: 10.7554/eLife.53473

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Gene (Salmonella enterica serovar Typhi) ttsA PMID: 23174673; MicrobesOnline Database sty1889
Gene (S. Typhi) ycbB PMID: 30250245;
MicrobesOnline Database
sty0997
Gene (Salmonella enteritidis) sen1395 PMID: 30250245; MicrobesOnline Database sen1395
Gene (S. Typhi) cdtB PMID: 18191792; MicrobesOnline Database sty1886
Strain, strain background (Escherichia coli) BL21 (DE3) Invitrogen Galan lab; electro competent
Strain, strain background (Escherichia coli) β−2163 Δnic 35 (DAP-) PMID: 15748991
Strain, strain background (Escherichia coli) B834 (DE3) Novagen Cat. No. 69041
Strain, strain background (Salmonella enterica serovar Typhi) ISP2825 PMID: 1879916
Antibody anti-FLAG M2 (Monoclonal mouse) Sigma-Aldrich 1:10000
Antibody anti-Salmonella O poly A-1 and Vi (Polyclonal rabbit) Becton, Dickinson and Co 1:10000
Antibody Alexa-Fluor 488-conjugated anti-mouse Invitrogen 1:2000
Antibody Alexa-Fluor 594-conjugated anti-rabbit Invitrogen 1:2000
Recombinant DNA reagent pSB 890 (plasmid) PMID: 7997169
Recombinant DNA reagent pET28b
(plasmid)
Invitrogen
Recombinant DNA reagent pSB 3783
(plasmid)
PMID: 30250245 pBAD24
Sequence-based reagent ttsAfor This paper PCR primers ATGACTAAAGATGAAATC
Sequence-based reagent ttsArev This paper PCR primers TTACAATCTTACCCGTTC
Sequence-based reagent sen1395for This paper PCR primers ATGAAACCGAAGGACGAA
Sequence-based reagent sen1395rev This paper PCR primers TCATATCAATACGCGCTC
Sequence-based reagent ycbBfor This paper PCR primers ATGTTGCTTAATAAGATG
Sequence-based reagent ycbBrev This paper PCR primers TTACCTGATTAATTGTTC
Software, algorithm ImageJ with Microbe J plug in http://rsbweb.nih.gov/ij/;
PMID: 27572972