Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Gene (Salmonella enterica serovar Typhi) | ttsA | PMID: 23174673; MicrobesOnline Database | sty1889 | |
Gene (S. Typhi) | ycbB | PMID: 30250245; MicrobesOnline Database |
sty0997 | |
Gene (Salmonella enteritidis) | sen1395 | PMID: 30250245; MicrobesOnline Database | sen1395 | |
Gene (S. Typhi) | cdtB | PMID: 18191792; MicrobesOnline Database | sty1886 | |
Strain, strain background (Escherichia coli) | BL21 (DE3) | Invitrogen | Galan lab; electro competent | |
Strain, strain background (Escherichia coli) | β−2163 Δnic 35 (DAP-) | PMID: 15748991 | ||
Strain, strain background (Escherichia coli) | B834 (DE3) | Novagen | Cat. No. 69041 | |
Strain, strain background (Salmonella enterica serovar Typhi) | ISP2825 | PMID: 1879916 | ||
Antibody | anti-FLAG M2 (Monoclonal mouse) | Sigma-Aldrich | 1:10000 | |
Antibody | anti-Salmonella O poly A-1 and Vi (Polyclonal rabbit) | Becton, Dickinson and Co | 1:10000 | |
Antibody | Alexa-Fluor 488-conjugated anti-mouse | Invitrogen | 1:2000 | |
Antibody | Alexa-Fluor 594-conjugated anti-rabbit | Invitrogen | 1:2000 | |
Recombinant DNA reagent | pSB 890 (plasmid) | PMID: 7997169 | ||
Recombinant DNA reagent | pET28b (plasmid) |
Invitrogen | ||
Recombinant DNA reagent | pSB 3783 (plasmid) |
PMID: 30250245 | pBAD24 | |
Sequence-based reagent | ttsAfor | This paper | PCR primers | ATGACTAAAGATGAAATC |
Sequence-based reagent | ttsArev | This paper | PCR primers | TTACAATCTTACCCGTTC |
Sequence-based reagent | sen1395for | This paper | PCR primers | ATGAAACCGAAGGACGAA |
Sequence-based reagent | sen1395rev | This paper | PCR primers | TCATATCAATACGCGCTC |
Sequence-based reagent | ycbBfor | This paper | PCR primers | ATGTTGCTTAATAAGATG |
Sequence-based reagent | ycbBrev | This paper | PCR primers | TTACCTGATTAATTGTTC |
Software, algorithm | ImageJ with Microbe J plug in |
http://rsbweb.nih.gov/ij/; PMID: 27572972 |