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. 2019 Jun 24;16(3):512–530. doi: 10.1080/15548627.2019.1630222

Figure 1.

Figure 1.

Chronic restraint stress (CRS)-induced reduction in the number of adult hippocampal NSCs is prevented by Atg7 deletion. (A) Scheme of mouse breeding and experimental time line for generation of tamoxifen (TAM)-inducible NSC-specific Atg7-NSC cKO mice. (B) Gene dose–dependence of ATG7 immunofluorescence intensities in the DG of the hippocampus. Scale bar: 50 μm. Solid and dotted circles indcate granule neurons and NSCs, respectively. The graph shows quantification of SOX2+ and ATG7+ cells (n = 6). (c) Timeline of the experiment. (D) Representative images of BrdU and SOX2 staining in the subgranular zone (SGZ) of the hippocampus. Scale bar: 20 μm. The graph on the right shows quantification of BrdU+ and SOX2+ cells (n = 6–8 per group). (E) Immunofluorescence of SOX2, NES and VIM in the SGZ of the DG of the hippocampus. Scale bar: 50 μm. Arrows indicate NSCs positive for each marker. ***P < 0.001 for the total SOX2+ cells. ###P < 0.001 for BrdU+ SOX2+ cells. n.s., not significant.