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. Author manuscript; available in PMC: 2020 Feb 4.
Published in final edited form as: Eur J Immunol. 2019 May 14;49(6):918–927. doi: 10.1002/eji.201847851

Figure 2.

Figure 2.

Role of chitin synthases in A. fumigatus survival in corneal infection. (A) Human neutrophils incubated 16 h with either WT Ku80, chitin synthase Family 1 mutants ΔChsA/B/C/G, or Family 2 mutants ΔChsE/Eb/D/F. Fungal viability was measured using the XTT viability dye. (B) The 48 h infected eyes were dissected and plated onto SDA agar plates overnight to determine CFU (corneas are 3 mm diameter). (C) Representative images of 24 h and 48 h infected corneas from C57BL/6 mice with Ku80, ΔChsA/B/C/G, or ΔChsE/Eb/D/F strains. (D) Quantification of percentage corneal opacity in 24 h and 48 h infected corneas. p values were determined following ANOVA analyses and a Tukey posttest. * = <0.05, ** = <0.001, *** = <0.0001. (A) Data are representative of two donors; (B, D) one representative experiment of three repeat experiments using 5–10 mice per group in each experiment where each data point represents an individual infected cornea.