Table 1.
Primer sequences
| Gene | Forward (5ʹ-3ʹ) | Reverse (5ʹ-3ʹ) |
|---|---|---|
| β-actin (mouse) | GTCCACCCCGGGGAAGGTGA | AGGCCTCAGACCTGGGCCATT |
| FAS (mouse) | GGCCAGTGCTATGCTGAGAT | CACTTCTGGAGACATCGCAAAC |
| CD36 | ATGGGCTGTGATCGGAACTG | TTTGCCACGTCATCTGGGTTT |
| ACCα (mouse) | TATTCGGCTGAAGCTGGTGTAC | TTCTTGCGATACACTCTGGTGC |
| TGF-β1 | TGTGTTGGTTGTAGAGGGCAAGGA | TTTGGAGCCTGGACACACAGTACA |
| COL-1 | GCAGGAGGTTTCGGCTAAGT | GCAACAAAGTCCGCGTATCC |
| FN | TAGCCCTGTCCAGGAGTTCA | CTGCAAGCCTTCAATAGTCA |
| Nrf2 | CAAACATTCAAGCCGATAGG | CGGCAACTTTATTCTTCCCTCT |
| NOX4 | TGCCTGCTCATTTGGCTGT | CCGGCACATAGGTAAAAGGATG |
| NOX2 | ACCTTACTGGCTGGGATGAA | ATTCTGGTGTTGGGGTGTTG |
| β-actin (human) | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT |
| PPARγ | GGGATCAGCTCCGTGGATCT | TGCACTTTGGTACTCTTGAAGTT |
| FAS (human) | ATGTCAGTCACTTGGGCATTA | CATCTGGACCCTCCTACCTCT |
| ACCα (human) | ATGTCTGGCTTGCACCTAGTA | CCCCAAAGCGAGTAACAAATTCT |
| SREBP-1c | CGGAACCATCTTGGCAACAGT | CGCTTCTCAATGGCGTTGT |
| PPARα | CTGGCATTTGTTTCTGTTCTTT | CTCCTCGGTGACTTATCCTGT |
| CPT-1 | TCCAGTTGGCTTATCGTGGTG | TCCAGAGTCCGATTGATTTTTGC |
| U6 | TGGCCCCTGCGCAAGGATG | Universal reverse primer (TIANGEN) |
| miR-99a-5p | AACCCGUAGAUCCGAUCUUGUG | Universal reverse primer (TIANGEN) |