Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 24;12(1):1717265. doi: 10.1080/19420862.2020.1717265

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Characterization of full-length anti-integrin-α11/β1 immunoglobulins. (a) Binding of anti-integrin-α11/β1 IgGs and a negative control IgG (x-axis) to C2C12 cells engineered to express the indicated integrins, assessed by flow cytometry fluorescence (y-axis). Data are shown for single-point measurements, and error bars indicate SD of two independent experiments. (b) Dose response curves for anti-integrin-α11/β1 IgGs and a negative control IgG (x-axis) binding to CAF094-α11/β1 or C2C12-α11/β1 cells, assessed by flow cytometry fluorescence (y-axis). Mean fluorescence intensity signals were normalized to the highest concentration value for each sample, and error bars indicate SD of two independent experiments. (c) Peptide counts for IP-MS analysis of CAF094-α11/β1 cell lysates immunoprecipitated with anti-integrin-α11/β1 IgGs or a negative control IgG. (d) Blocking of anti-integrin-α11/β1 IgGs (x-axis) binding to CAF094-α11/β1 cells by indicated Fabs, assessed by flow cytometry fluorescence (y-axis). Data are shown for single-point measurements, and error bars indicate SD of two independent experiments.