(
a) Plot showing differentially expressed genes between cluster 7 versus cluster 2 cells. Differentially expressed genes (p-value<0.05) are highlighted in red (upregulated in cluster 7 cells) and blue (upregulated in cluster 2 cells). Complete gene list can be found in
Figure 1—source data 3. 771 genes were differentially expressed (p<0.05; adjusted p-value after Benjamini-Hochberg correction), of which 752 were specifically upregulated in cluster seven cardiomyocytes, including the border zone genes,
nppa,
nppb and
mustn1b. (
b) GO-terms for upregulated genes in cluster 7 and cluster two respectively (p<0.01). (
c) Gene set enrichment analysis for glycolysis genes on all genes with differential expression between cluster 7 versus cluster 2 cells (p<0.05). (
d) Log2 fold change of differentially expressed genes (cluster 7 vs cluster 2) with a function in energy metabolism. High relative expression in cluster seven depicted in red, high expression in cluster two depicted in blue. In situ hybridization for the glycolysis gene
hexokinase1 (a,b) and the embryonic cardiac gene myomesin1b (c,d) on sections of injured zebrafish hearts at three dpi (a,c) and seven dpi (b,d). While
hk1 expression in border zone cardiomyocytes was visible at 3 dpi and seven dpi,
myom1b expression was visible at seven dpi but undetectable at 3dpi. (a’,b’,c’ and d’) show zoom-in of boxed areas in corresponding panels. Dashed line indicates the injury site.