Figure 1:
Characterization of G-rich and pyrimidine-rich sequences from tRNA digests by tandem mass spectrometry. Sequence informative fragment ion series (cn, with common 5’-end and yn with common 3’-end) are illustrated in the spectra. (A) CID spectrum of MC1 digestion product with m/z 1316.82 corresponding to [D]GGGAGAGCGCC>p from tRNAAla-[cmo5U]GC. (B) CID spectrum of MC1 digestion product with m/z 1215.32 corresponding to [D]GGGAGAGCGC>p from tRNAAla-GGC. (C) CID spectrum of cusativin digestion product with m/z 1249.4 corresponding to AAGUCCCCCCCC>p from tRNALeu-CAG.