Fig. 3.

Overexpression of HIF-1α in the parental cells and study of their radioresistance. a Western blot analysis for detection of HIF-1α overexpression in Hela, SAS, and HepG2. The cells were transfected with an empty vector (EV), that containing HIF-1α or HIF-1α-3A. β-Actin was used for internal control. b Cells stably transfected with HIF-1α-3A underwent normoxic and hypoxic conditions. Under hypoxic HIF-1α expression was induced in control cells transfected with an empty vector. However, a high level of expression of HIF-1α was observed in the cells transfected with the 16 construct containing HIF-1α -3A under normoxia condition as well. β-actin was used as loading control. c Survival assay of the parental cells in which HIF-1α or HIF1α -3A was overexpressed as described above. HIF-1α -3A protected cells against irradiation. CI, HepG2; CII, SAS; CIII, Hela; EV, empty vector; − , normoxia; +, hypoxia (mean ± SD, number of samples = 3, **p ≤ 0.01)