Fig. 4.

Downregulation of HIF-1α in CRR cells and survival assay. a Western blot analysis for detection of down regulation of HIF-1α in Hela-R, HepG2-8960-R, and SAS-R1 cells. β-actin was used as an internal control. B; Survival assay in CRR cells transfected with HIF-1α shRNA or control shRNA. Cells exposed to different doses of irradiation. Downregulation of HIF-1α had no significant effects in the CRR cells in terms of sensitization to irradiation except HepG2 (mean ± SD, number of samples = 3, *p ≤ 0.5)