Mechanism of Reconstitution/Activation of the Soluble PQQ-Dependent Glucose Dehydrogenase from Acinetobacter calcoaceticus: A Comprehensive Study

Abstract

The ability to switch on the activity of an enzyme through its spontaneous reconstitution has proven to be a valuable tool in fundamental studies of enzyme structure/reactivity relationships or in the design of artificial signal transduction systems in bioelectronics, synthetic biology, or bioanalytical applications. In particular, those based on the spontaneous reconstitution/activation of the apo-PQQ-dependent soluble glucose dehydrogenase (sGDH) from Acinetobacter calcoaceticus were widely developed. However, the reconstitution mechanism of sGDH with its two cofactors, i.e., pyrroloquinoline quinone (PQQ) and Ca²⁺, remains unknown. The objective here is to elucidate this mechanism by stopped-flow kinetics under single-turnover conditions. The reconstitution of sGDH exhibited biphasic kinetics, characteristic of a square reaction scheme associated with two activation pathways. From a complete kinetic analysis, we were able to fully predict the reconstitution dynamics and also to demonstrate that when PQQ first binds to apo-sGDH, it strongly impedes the access of Ca²⁺ to its enclosed position at the bottom of the enzyme binding site, thereby greatly slowing down the reconstitution rate of sGDH. This slow calcium insertion may purposely be accelerated by providing more flexibility to the Ca²⁺ binding loop through the specific mutation of the calcium-coordinating P248 proline residue, reducing thus the kinetic barrier to calcium ion insertion. The dynamic nature of the reconstitution process is also supported by the observation of a clear loop shift and a reorganization of the hydrogen-bonding network and van der Waals interactions observed in both active sites of the apo and holo forms, a structural change modulation that was revealed from the refined X-ray structure of apo-sGDH (PDB: 5MIN).

Introduction

Many enzymes require the noncovalent insertion of cofactors (e.g., flavins, hemes, metal ions, iron–sulfur clusters) into their apoprotein binding site to be fully active. In numerous cases, this enzyme activation process, also termed enzyme reconstitution, can be achieved in vitro through a precise control of the reaction conditions (temperature, buffer composition, pH, etc.), paving thus the way for fundamental investigations of enzyme structure/reactivity relationships (notably by taking advantage of engineered binding partners such as apoenzyme mutants and/or cofactor analogues)¹⁻⁸ or even for discovering new enzyme functionalities.⁹⁻¹¹ Besides to the capacity of switching on the activity of an enzyme by simply adding its cofactor, enzyme reconstitution has also been advantageously exploited in different biotechnological applications,^5,10,12−19 ranging from the design of artificial signal transduction systems for analytical purposes to the development of new applications in biotechnology, bioelectronics, or synthetic biology.^10,20−25

Among the enzymes whose catalytic activity can be easily and efficiently switched on via reconstitution, the soluble quinoprotein glucose dehydrogenase (sGDH, code UniprotKB F0KFV3) is certainly the most prevailing and attractive.^15,26−33 The reason for such interest is that, in the presence of calcium ions, the catalytic property of sGDH for aldoses oxidation can be rapidly and spontaneously activated through the specific and tight binding of its PQQ cofactor to the apoprotein (apo-sGDH). This property has led to the design of novel analytical methods for the sensitive detection of calcium ions³⁰ or PQQ²⁹ and to the conception of unique signal amplification strategies to boost the analytical performances of miscellaneous affinity binding assays.^15,31,32 Other features that make sGDH an attractive activatable enzyme is the ease with which the apoenzyme can be overproduced in a recombinant strain of Escherichia coli and isolated with a high yield and purity (totally free of PQQ).^34,35 The reconstitution reaction is furthermore spontaneous and fast in the presence of Ca²⁺, mainly driven by the high-affinity binding of PQQ to the apo-sGDH (equilibrium dissociation constant in the sub-nanomolar range).²⁹ Also, the holoenzyme exhibits a remarkably high catalytic activity toward the oxidation of glucose with concomitant reduction of a wide range of natural or artificial electron acceptors, a catalytic reactivity that can be easily monitored either spectrophotometrically or electrochemically. Albeit there are several works reporting on sGDH reconstitution^34,36−38 as well as on its exploitation in different biotechnological applications,^15,29−32 its reconstitution mechanism remains unknown. Previous studies have yet established that, similarly to alcohol dehydrogenase, reconstitution of the homodimeric sGDH enzyme requires the binding of six calcium ions and two PQQ molecules.³⁹⁻⁴¹ Four of the Ca²⁺ ions are involved in the functional dimerization of the two protein subunits (i.e., held together by four Ca²⁺ ions shared at the interfaces) and two for the activation of each PQQ cofactor present in each of two apoenzyme subunits (i.e., one Ca²⁺ ion per PQQ in close interaction within the binding site).³⁶ Other divalent cations (Cd²⁺, Mn²⁺, Sr²⁺) were also revealed to be effective in the dimerization and activation of the apoenzyme.³⁷ In addition, the binding of PQQ was shown to be remarkably efficient, not only thermodynamically but also kinetically. Dissociation constants in the nano- to picomolar range were reported in the presence of millimolar Ca²⁺, demonstrating the high-affinity binding of PQQ to the apo-sGDH,^29,37 while a second-order rate constant of (∼1–2) × 10⁶ M^–1 s^–1 characterizing the binding of PQQ to the apo-sGDH was determined for the enzyme either reconstituted in a homogenous solution⁴² or once immobilized on an electrode surface.²⁹ These kinetics were, however, examined in the presence of a large excess of Ca²⁺ and under steady-state kinetics, preventing thus a detailed understanding of the role played by this second cofactor in the reconstitution mechanism.

The aim of this paper is thus to fill this gap by investigating the reconstitution mechanism of apo-sGDH with PQQ and Ca²⁺ by stopped-flow kinetics. From analysis of the transient kinetics under single-turnover conditions, we show that the reconstitution follows two different pathways, each depending on the order the cofactors are positioned in the binding site, i.e., PQQ binding followed by Ca²⁺ or Ca²⁺ binding followed by PQQ. It finally allows us to propose a comprehensive overview of the reconstitution mechanism of sGDH, from which we were then able to quantitatively rationalize and predict the reconstitution dynamics. Moreover, from the determination of the complete X-ray structure of apo-sGDH and comparison to the holo-sGDH, we were able to evidence a movement of the loop located near the binding pocket, highlighting thus the dynamic nature of the reconstitution process.

Results

As previously reported by Duine and colleagues,^34,36 the isolated apo-sGDH from E. coli recombinant strain is in the dimeric form, containing thus Ca²⁺ at the interface between the two subunits but no PQQ. The preparation also remains dimeric after desalting over gel filtration or upon high dilution in a Ca²⁺-free buffer. Even in the presence of an excess of ethylenediaminetetraacetic acid (EDTA), the protein remains as a dimer, demonstrating that Ca²⁺ once bounded at the subunit interface is locked.

The kinetics of sGDH reconstitution was investigated by stopped-flow experiments. The methodology consists of rapidly mixing PQQ, apo-sGDH, Ca²⁺, and excess glucose and then following by UV–visible spectroscopy the transient reduction of PQQ once the latter is properly bounded and activated into the apoenzyme binding site. In this way, single-turnover reduction of PQQ witnesses the active behavior of the reconstituted enzyme since free PQQ cannot be directly chemically reduced by glucose in solution. The reduction rate of the PQQ cofactor in the enzyme was monitored at λ = 338 nm, a wavelength that relates to a maximal absorbance change between the oxidized (i.e., holo_ox) and reduced state of sGDH (i.e., holo_red wherein the PQQ cofactor is doubly reduced in its pyrroloquinoline quinol form or PQQH₂) (Figure S1 in the Supporting Information). For the kinetic information of the enzymatic reconstitution process to be properly extracted, it was indispensable to select experimental conditions that lead to an almost instantaneous reduction of the bounded (and activated) PQQ cofactor relative to the rate of its upstream equilibrium binding to the apoenzyme binding site (in other words, to select conditions where the reduction of the holo_ox by glucose is not rate-limiting). To evaluate how fast is the reduction rate of the wild-type holo_ox by an excess glucose, we mixed 2.5 μM subunits of native sGDH with 100 μM glucose in a stopped-flow apparatus and monitored the transient kinetics by UV–visible spectroscopy at 338 nm (Figure S2). The fast absorbance change that occurs at 338 nm over a maximal variation of ΔA_max ∼ 0.05 (corresponding to a full reduction of sGDH) and within a time scale shorter than 30 ms allows setting the time window required for complete enzyme reduction by glucose (it is worth to note that because of the fast rate of sGDH reduction a large fraction of the absorbance change was completed during the dead time of the instrument). From the known maximal absorbance change at 338 nm and the fit of an exponential law to the experimental data, an apparent first-order rate constant of 130 ± 2 s^–1 was found. This value agrees well with that previously determined for the reductive half-reaction of sGDH^39,42 under similar conditions, confirming that the catalyzed hydride transfer reaction from glucose to the PQQ cofactor in the active site is a fast process. These experimental conditions (i.e., 2.5 μM subunits of sGDH and 100 μM glucose), finally leading to a fast reduction of holo_ox by glucose, were then selected for the further kinetic experiments.

To investigate the kinetics of sGDH reconstitution under single-turnover conditions, we tested PQQ concentrations ranging from substoichiometric to overstoichiometric ratios (i.e., from 0.5 to 40 μM) relative to the total amount of available apo-sGDH binding sites (2.5 μM subunits) in the presence of fixed concentrations of CaCl₂ (3 mM) and glucose (100 μM). The data reported in Figure 1A exhibit biphasic exponential kinetics within a time frame extending from 20 ms to 100 s, much slower than the time scale required for the above reduction of the holoenzyme by glucose (half-life t_1/2 ∼ 5 ms, Figure S2). This therefore means that under the selected conditions, the reaction is essentially rate-controlled by the equilibrium binding of PQQ to the apoenzyme and not by the following step of holoenzyme reduction by glucose. This result also shows that the proposed transient UV–visible kinetic methodology is relevant for characterizing the reconstitution mechanism of sGDH.

Figure 1.

Stopped-flow transient traces of the catalytic reduction of PQQ by glucose during the reconstitution/activation of apo-sGDH into holo-sGDH. (A) Kinetic traces (average of four consecutive experiments) obtained after mixing 2.5 μM subunits of apo-sGDH with 100 μM glucose, 3 mM CaCl₂, and different concentrations of PQQ (from left to right): 40, 25, 15, 10, 5, 2.5, 2, 1.5, 1, and 0.5 μM (the code color for each [PQQ] is reported on the graph). All concentrations given are after mixing. The reactions were monitored at 338 nm and 10 °C in a 50 mM Tris buffer (pH 7.5). (B) Titration plot of apo-GDH by PQQ obtained from the plot of total absorbance change in (A) as a function of the [PQQ]/[apo-sGDH] ratio. (C) Kinetic traces (average of four consecutive experiments) obtained after mixing 2.5 μM subunits of apo-sGDH with 5 μM PQQ (2-fold excess), 100 μM glucose, and different concentrations of CaCl₂: 9 μM, 100 μM, 3 mM, 30 mM, 60 mM, and 150 mM (the code color for each [CaCl₂] is reported on the graph). All concentrations given are after mixing, and other experimental conditions are the same as in (A). The black dotted curves in (A) and (C) are the best fits of a biexponential law to the experimental data.

The maximal amplitude of the absorbance change at 338 nm (ΔA_max) in Figure 1A is linked to the fraction of apo-sGDH that reconstitutes and activates. By reporting this maximal amplitude as a function of the [PQQ]/[apo-sGDH] ratio, we can obtain the titration plot of apo-sGDH by PQQ (Figure 1B). The plot shows that apo-GDH can be fully reconstituted (and so fully activated) at a 1.1:1 ratio of PQQ/apoenzyme subunits, which is, within the experimental error, close to that expected for the binding of one PQQ molecule per subunit. Consequently, the two well-distinct kinetic phases observed in Figure 1A suggest the existence of two different enzyme activation pathways, occurring in parallel but at different paces.

The kinetics of sGDH reconstitution was also examined for different concentrations of calcium (ranging from 9 μM to 150 mM), keeping constant the ratio of PQQ/apo-sGDH subunits to an excess of 2 (Figure 1C). The gradual transition from a biphasic kinetics at low calcium concentrations (for which the slow kinetic pathway predominates) to a simple first-order kinetics at high Ca²⁺ concentration provides evidence that calcium plays a key role in the partitioning of reactants between the two parallel pathways. In addition, the almost identical values of the maximal absorbance change recorded at the end of kinetics (ΔA_max ∼ 0.05) are in good agreement with the expected burst amplitude for a quantitative activation of the 2.5 μM subunits of apo-sGDH present in solution.

Given that both PQQ and Ca²⁺ are required for enzyme activation, the reconstitution mechanism can thus be reasonably described by a square reaction scheme with two parallel pathways in competition, each depending on the binding order of the two entities to form an activated ternary complex (i.e., holo_ox), which then rapidly reduces to holo_red in the presence of glucose (Scheme 1). From the amplitude of the absorbance change of one phase (ΔA₁ or ΔA₂) relative to the total absorbance change (ΔA_max = ΔA₁ + ΔA₂), these experiments give an access to the relative population of the different transient species contributing to each reaction path. The amplitudes ΔA₁ and ΔA₂ as well as the observed rates k_1,obs and k_2,obs associated with each kinetic phase were recovered from the nonlinear regression fit of a biexponential function to the kinetic traces as shown in Figure 1A,C (see Tables S1 and S2 for the extracted data). Under the reaction conditions of 3 mM Ca²⁺ (Figure 1A), the ratio ΔA₁/ΔA_max is almost the same regardless of PQQ concentration and close to an average value of 0.6 (Table S1), a result that thus provides evidence for a roughly constant balanced initial distribution of sGDH between two populations that can each independently reconstitute according to a fast or a slow pathway. This partitioning between fast and slow reconstitutions is also strongly dependent on [Ca²⁺], as attested by the plot of ΔA₁ or ΔA₂ as a function of calcium concentration in Figure S3 (the graph was obtained from the values reported in Table S2 as well as from those collected from other experiments), showing an hyperbolic dependence of the relative amplitude of each phase on [Ca²⁺]. This behavior provides evidence for a fast equilibrated binding reaction between apo-sGDH and Ca²⁺ (leading to the formation of apo-sGDH/Ca²⁺), fast enough to be always assumed at equilibrium before PQQ significantly binds to the protein. From the fit of a standard binding isotherm to the data (Figure S3), an apparent equilibrium dissociation constant of K_d^apo-sGDH/Ca = 2.0 ± 0.50 mM was recovered.

Scheme 1. Square Scheme Reconstitution Mechanism of apo-sGDH with the Random Binding of Ca²⁺ and PQQ To Form the Ternary Activated Complex holo_ox, Which Then Rapidly Converts into holo_red in the Presence of Glucose.

The values k_1,obs and k_2,obs gathered in Tables S1 and S2 are plotted in Figure 2 as a function of PQQ concentration. In conditions of pseudo-first-order (ratio [PQQ]/apo-sGDH ≥ 2), the observed rate k_1,obs shows a linear dependency on [PQQ], suggesting that the fast phase of enzyme reconstitution is rate-limited by the entrance of PQQ. In contrast, k_2,obs is found independent of [PQQ] (an average value of 0.2 s^–1) over the whole explored range (0.5–40 μM), demonstrating that PQQ is not the rate-limiting factor in this slow enzyme reconstitution pathway. Interestingly, the observed rates were also found independent of the order the three partners were mixed inside the two-syringe stopped-flow device (Table S4), showing similar values of k_1,obs and k_2,obs whether the content of the syringe loaded with apo-sGDH or PQQ was preincubated or not with Ca²⁺. This observation reinforces the hypothesis that a fast equilibrium binding occurs between apo-sGDH and Ca²⁺, fast enough to be nearly always at equilibrium during the whole enzyme kinetic experiments.

Figure 2.

Plots of (red dots) k_1,obs and (green squares) k_2,obs (both recovered from the fits of a biexponential function to experimental kinetic curves shown in Figure 1A and whose data are compiled in Table S1) as a function of the PQQ concentration or [PQQ]/[apo-sGDH] ratio. The straight line is the linear regression fit to the k_1,obs data determined under pseudo-first-order conditions in the apoenzyme (i.e., [PQQ] ≥ 5 μM). The slope of the linear regression fit allows the calculation of the bimolecular rate constant of the reaction between PQQ and apo-sGDH, leading to a value of k_1,PQQ = (1.08 ± 0.05) × 10⁶ M^–1 s^–1.

The linear relationship between k_1,obs and [PQQ] at high PQQ/apo-sGDH ratios (i.e., conditions of pseudo-first-order in the apoenzyme) gives an access to the second-order rate constant associated with the binding of PQQ to apo-sGDH under 3 mM Ca²⁺. From the linear regression fit in Figure 2 (red line), a k_1,PQQ value of (1.08 ± 0.05) × 10⁶ M^–1 s^–1 is obtained. This value was confirmed from the analysis of a larger set of kinetic experiments performed under pseudo-first-order conditions (see Figure S4), leading to k_1,PQQ = (1.12 ± 0.01) × 10⁶ M^–1 s^–1.

It was previously shown by Duine and colleagues³⁷ that the addition of PQQ to apo-sGDH could be monitored from the quenching of fluorescence engendered by the six tryptophan residues present in the protein. We therefore took advantage of this quenching of fluorescence to study the kinetics of sGDH reconstitution. Figure S5 shows that the maximum of fluorescence emitted by apo-sGDH at 340 nm (red curve) is strongly quenched after the addition of PQQ together with Ca²⁺ ions (blue curve). Stopped-flow experiments were thus performed at 340 nm by monitoring the quenching rate of the apo-sGDH fluorescence as a function of [PQQ] and [Ca²⁺] (only overstoichiometric ratios of [PQQ]/[apo-sGDH] were considered because of a too weak and noisy fluorescence under substoichiometric conditions) (Figure 3). In contrast to the above kinetic traces determined from the absorbance change at 338 nm, the time-course plots of quenching of fluorescence in Figure 3A,B are all well-characterized by a single kinetic phase, whatever the concentration of PQQ and Ca²⁺. From the fit of a monoexponential decay to the highest PQQ concentrations, the recovered observed rate not only linearly depends on the PQQ concentration (Figure S4, red triangles) but also coincides with the values of k_1,obs previously determined from the absorbance change (it is to note that the significant change observed for both the fluorescence offset and the overall fluorescence amplitude as a function of PQQ concentration is presumed to be associated with a fast nonspecific quenching of fluorescence generated by excess PQQ in solution). This result strongly supports that the quenching of fluorescence of apo-sGDH in the absence of glucose addresses the same fast kinetic process than the one associated with the fast phase of the absorbance change at 338 nm in the presence of glucose, a fast process we have attributed to the entrance of PQQ in the apo-sGDH binding site. From the slope of the linear regression fit to the data in Figure S4, a second-order rate constant of (1.25 ± 0.03) × 10⁶ M^–1 s^–1 was obtained, which is thus close to the one obtained from the absorbance change at 338 nm (i.e., (1.12 ± 0.01) × 10⁶ M^–1 s^–1).

Figure 3.

Transient traces of the quenching of tryptophan fluorescence residues of apo-sGDH during enzyme reconstitution with PQQ and calcium. (A) Stopped-flow kinetics obtained after mixing 2.5 μM subunits of apo-sGDH with 3 mM CaCl₂ and different concentrations of PQQ (from left to right): 40, 25, 15, 10, 5, and 2.5 μM. All concentrations given are after mixing. The reactions were carried out at 10 °C in a 50 mM Tris buffer (pH 7.5), and the fluorescence was monitored at 340 nm with an excitation at 297 nm. (B) Stopped-flow kinetics obtained after mixing 2.5 μM subunits of apo-sGDH with 5 μM PQQ and different concentrations of CaCl₂ (from left to right): 9 μM, 100 μM, 3 mM, 30 mM, 60 mM, and 150 mM. All concentrations given are after mixing, and other experimental conditions are the same as in (A). The black dotted curves in (A) and (B) are the best fits of a monoexponential function to the experimental data.

Concerning the effect of [Ca²⁺] on the time course of fluorescence quenching (performed here at a fixed [PQQ]/[apo-sGDH] ratio of 2), the kinetic plots in Figure 3B show that k_obs remains relatively constant and independent of [Ca²⁺] (k_obs ∼ 4–5 s^–1) until the latter remains lower than 3 mM, while it progressively decreases for Ca²⁺ concentrations >3 mM (Table S3). This outcome correlates very well with the fast kinetic phase of the absorbance change at 338 nm, wherein k_1,obs is similarly slowed down at [Ca²⁺] > 3 mM (the kinetic traces performed at 30, 60, and 150 mM Ca²⁺ in Figure 3B matched quite well those reported in Figure 1C at the same Ca²⁺ concentrations). As will be discussed later on, we have attributed this behavior to an inhibition of the fast enzyme reconstitution pathway by excess Ca²⁺. Additionally, the fact that the kinetics of fluorescence quenching follows a single exponential decay independent of [Ca²⁺] for concentrations lower than 3 mM provides evidence that the surveyed process under these conditions is the conformational changes induced by the binding of PQQ to the apoenzyme site (and this, whether or not there is a Ca²⁺ ion present in the binding site). Such a behavior clearly differs from the dependence of k_1,obs on [Ca²⁺] observed in Figure 1C and Table S2, which likewise addresses the binding of PQQ to the apo-sGDH but differently since in this case this is the fraction of apo-sGDH (i.e., apo-sGDH/Ca²⁺) that is involved in the fast activation pathway (a fraction that itself depends on the calcium concentration through the fast equilibrated reaction between apo-sGDH and Ca²⁺, leading thus to a dependence of k_1,obs on [Ca²⁺] at low Ca²⁺ concentrations).

Duine and his collaborators have demonstrated that, in the absence of calcium ions, apo-GDH can reconstitute into a much less active form of sGDH, baptized holoX_ox, a Ca²⁺-free enzyme able to slowly convert into its reduced holoX_red counterpart in the presence of glucose.^34,36 To characterize the kinetics of these two processes (i.e., the rate of holoX_ox formation as well as the rate of the slow holoX_ox reduction by glucose in the absence of calcium), we performed stopped-flow kinetics experiments without calcium and in the presence of a large excess of EDTA (to scavenge any residual traces of free Ca²⁺ in solution) (Figure S6). The conversion rate of apo-sGDH into holoX_ox was extracted from the quenching of fluorescence of tryptophan at 340 nm as a function of different concentrations of PQQ. The resulting kinetic traces in Figure S6 are characterized by an exponential decay of fluorescence, similar to that previously observed in the presence of calcium. The observed rate extracted from each plot varies linearly with excess PQQ (Figure S6), thus allowing to recover a second-order rate constant of k_2,PQQ = 0.94 × 10⁶ M^–1 s^–1. This value is very close to the rate k_1,PQQ determined in the presence of calcium, leading us to conclude that the binding rate of PQQ to the apoenzyme is almost unchanged whether there is or not a calcium ion anchored within the binding site of apo-sGDH.

To further characterize the reduction rate of holoX_ox into holoX_red subsequent to the addition of glucose, we monitored the reaction by UV–visible spectrophotometry at 307 nm (see Figure S7 for the selection of this wavelength) in a stopped-flow apparatus. The kinetic trace reported in Figure S8 demonstrates a very slow reduction rate of holoX_ox by glucose, leading to a pseudo-first-order rate of k_red,X = 8.4 × 10^–4 s^–1, a value that is more than 160 000 times slower than in the presence of calcium (k_red = 130 s^–1, Figure S2). This result confirms the strong effect of Ca²⁺ on the sGDH reactivity, the role of which is to activate the PQQ cofactor (activation at the C₅ position of PQQ) and to promote the hydride transfer reaction between glucose and PQQ.⁴³

The possibility to isolate the holoX_red form of sGDH has pushed us to determine the incorporation rate of Ca²⁺ into holoX_red to form the holo_red, a binding reaction wherein the reduced PQQ cofactor already present in the enzyme pocket is assumed to hinder the access of calcium, the latter position of which is located just behind the PQQ binding site at the bottom of the active site.⁴⁰ For such a purpose, we have monitored by stopped flow the transition from holoX_red to holo_red by simply mixing different concentrations of Ca²⁺ to a holoX_red solution, followed by the absorbance measurement at 338 nm. The reaction rate was observed to hyperbolically depend on the concentration of Ca²⁺ (Figure S9), suggesting a two-step binding reaction mechanism that we have assumed to obey Michaelis–Menten-type kinetics. From the best curve fitting, the following maximal rate (k_3,Ca^′) and apparent Michaelis–Menten constant (K_3,Ca) were recovered: k_3,Ca^′ = 0.52 ± 0.01 s^–1 and K_3,Ca = 5.30 ± 0.69 mM.

On account of the very slow reduction of holoX_ox into holoX_red and the fast and almost irreversible conversion of apo-sGDH into holoX_ox (the strong equilibrium binding constant is assumed to lead to a slow dissociation rate of PQQ from holoX_ox, vide infra), it is possible to characterize the binding reaction of Ca²⁺ to holoX_ox (in a similar way as above with holoX_red), generating thus the transient species holo_ox, which then rapidly converts into holo_red in the presence of glucose. For the rate of this reaction to be assessed, different concentrations of calcium in the presence of an excess of glucose were then mixed with holoX_ox in a stopped-flow apparatus and the reaction mixture was then kinetically monitored by UV–vis at 338 nm (Figure S10). The resulting kinetic plots were fitted to a monoexponential function, leading to an observed rate that hyperbolically depends on the concentration of Ca²⁺ (Figure S10). Again, this behavior suggests a two-step binding reaction mechanism that we have once more assumed to obey a Michaelis–Menten-type reaction. From the best curve fitting, the following parameters were obtained: k_2,Ca^′ = 1.19 ± 0.2 s^–1 and K_2,Ca = 0.6 ± 0.3 mM. The value of k_2,Ca^′ (that can formally be assimilated to k_2,obs) is here somewhat higher when compared with k_2,obs determined in Figure 2. We attributed this discrepancy to the high scattering we encountered in the experimental determination of k_2,Ca and K_2,Ca^M (Figure S10), probably resulting from the presence of a large excess of EDTA. In addition, one may note that the value of K_2,Ca is an order of magnitude lower than K_3,Ca^M, revealing the strongest interaction of calcium with the oxidized form of PQQ as compared to the reduced one.

To provide further evidence that the slow activation pathways in the sGDH reconstitution mechanism are linked to the difficulty for calcium to reach its enclosed position when PQQ is already present in the enzyme binding site (steric effect), we have mutated the P248 amino acid into alanine. The mutation of this proline was chosen because it is located within a loop near the active site that is expected to significantly contribute to the three-dimensional organization of the enzyme active site (the distinctive cyclic structure of the side chain of proline is well-known to provide a conformational rigidity compared to other amino acids, affecting thus the secondary structure of proteins near a proline residue⁴⁴). An additional reason is that the two carbonyl groups present on the amino acids P248 and G247 are known to directly interact with the calcium ion, which itself strongly interacts with PQQ (Figure 4D). The P248A mutation is thus anticipated to significantly increase the flexibility and mobility of the loop coordinating the calcium ion and therefore to potentially reduce the kinetic cost of the calcium insertion when PQQ binds first. The reconstitution of the mutated P248A apo-sGDH was then characterized by stopped-flow kinetic experiments under the same conditions as for the wild-type apoenzyme (Table S4). Similar to that of the wild-type enzyme, biphasic kinetic traces were obtained, leading for the fast phase to an observed rate constant of k_1,obs = 12 ± 2 s^–1 (Table S4), which is within a range of a factor 2, comparable to that obtained with the wild-type apo-sGDH (i.e., k_1,obs = 5.4 ± 0.9 s^–1). This result demonstrates a negligible effect of the mutation on the binding rate of PQQ. In contrast, the rate of the slow phase is observed to be significantly enhanced for the mutant enzyme (k_2,obs = 3.7 ± 0.8 s^–1, which is ∼16-fold higher than for the wild-type, i.e., k_2,obs = 0.23 ± 0.02 s^–1) (Table S4), suggesting that the P248A mutation has a substantial structural effect on the accessibility of Ca²⁺ to its enclosed position within the holoX active site. As anticipated, the mutation leads to an increased flexibility of the loop coordinating Ca²⁺, which thereby facilitates the incorporation and/or access of the calcium ion within the sterically congested site of holoX.

Figure 4.

(A) Superimposition of the X-ray structures of (red) apo-sGDH (PDB: 5MIN) and (green) holo-sGDH (PDB: 1QBI) in the loop region ASN334 to TRP346, showing the disulfide bridge (CYS345–CYS338) and the trace of the main chain (missing in 1QBI). (B) Superimposition of the Cα traces of apo-sGDH (5MIN, red), holo-sGDH (1C9U, green), and apo-sGDH (1QBI, blue) structures in the same region, showing the loop shifting (overall root mean square deviation 1.13, 1.9 Å for GLY339). The TRP346 (in the stick mode) position is well conserved. (C, D) Same views of the key residues and cofactors in the active site of (C) apo-sGDH (PDB: 5MIN) and (D) holo-sGDH (PDB: 1C9U), highlighting the different positions of the calcium atom in each structure and, in the case of the apo form, the water molecule network filling the cavity in the absence of PQQ.

Finally, with the aim to fully characterize the environment of the binding pocket before reconstitution, we have determined the X-ray structure of apo-sGDH in the presence of calcium at 1.76 Å of resolution (PDB 5MIN). In the previously published X-ray structure of apo-sGDH, two loops from positions 105 to 110 and 333 to 346 and a disulfide bridge (CYS345–CYS338) were lacking (PDB 1QBI),⁴¹ while in our new X-ray structure of apo-sGDH, the disulfide bridge is intact and the two loops are clearly defined (see Figure 4A,B for the loop from positions 334 to 346 including the disulfide bridge and Figure S11 to see the electron density of the loop from positions 333 to 346). Interestingly, the superimposition of the apo and holo Cα traces shows a shift of 1 to 2 Å in the relative position of the loop located from 333 to 346 (Figure 4B). This clearly suggests that a local conformational rearrangement occurs during and/or following the binding of PQQ, which might act as a lock to ensure the strong affinity of PQQ (a dissociation constant of 30 pM and a dissociation rate as low as k_–1,PQQ = 3.4 × 10^–5 s^–1, vide infra). This might then result in a kinetic barrier (or a steric constraint) to overcome for the insertion of Ca²⁺ when PQQ is already in place in the apo-sGDH binding site.

Discussion

On the basis of the overall results obtained above, we can finally propose a more elaborate version of the minimum square reaction mechanism described in Scheme 1 by the mechanism depicted in Scheme 2. The latter involves a preferred-order binding of the two cofactors rather than a completely random mechanism (a preferred-order binding that strongly depends on the calcium concentration) to form, on the one side (a fast pathway), a ternary holo_ox complex that rapidly converts into its reduced holo_red form in the presence of glucose and, on the other side (a slow pathway), a binary holoX_ox complex that can either (i) be slowly converted in the presence of Ca²⁺ into a ternary one according to a two-step reaction (assumed here to be analogous to a Michaelis–Menten-type process, wherein Ca²⁺ reacts first through a fast equilibrium binding followed by a slow first-order reaction to lock Ca²⁺ in its sterically congested site in the enzyme), followed by fast reduction with glucose, or (ii) be directly very slowly reduced by glucose into holoX_red before being definitely converted into holo_red through a two-step reaction with Ca²⁺ (that we have again considered as a Michaelis–Menten type process in agreement with the hyperbolic rate dependence on [Ca²⁺]). In addition, to take into account the reversible apparent inhibition observed at high Ca²⁺ concentrations (>3 mM) (i.e., the decrease of k_1,obs at high [Ca²⁺]; see Table S3), an additional equilibrium reaction was added (the inhibition path) by considering the fast reversible formation of a PQQ/Ca²⁺ complex, which thus consequently decreases the amount of free PQQ in solution and slow-downs (or inhibits) the enzymatic reconstitution at very high [Ca²⁺]. Although there is no information about the real nature of this inhibition process, we have postulated that it may arise from an equilibrium binding interaction between PQQ and Ca²⁺ (with kinetic rate constants k_i and k_–i), leading thus to a PQQ/Ca²⁺ complex ineffective in reconstituting apo-sGDH into a holo active form. The interaction between PQQ and Ca²⁺ is supported by previous works where it has been shown that a PQQ/Ca²⁺ complex can be generated at high Ca²⁺ concentration in either an organic solvent⁴⁵ or an aqueous buffer.⁴⁶

Scheme 2. Global Mechanism for the Reconstitution/Activation of apo-sGDH into Active PQQ-sGDH.

A full kinetic description of the global reconstitution mechanism in Scheme 2 implies the definition of 14 rate constants, which have to be as far as possible independently determined. Let us first focus on the fast pathway. Five rate constants are required for this pathway: two for the reversible binding of Ca²⁺ to apo-sGDH (i.e., k_1,Ca and k_–1,Ca for the association and dissociation rate constants of Ca²⁺, respectively), two for the binding of PQQ to the apo-sGDH/Ca²⁺ complex (i.e., k_1,PQQ and k_–1,PQQ for the association and dissociation rate constants of PQQ, respectively), and one for the reduction of holo_ox to holo_red at a saturated concentration of glucose (i.e., k_red = 130 s^–1). As discussed earlier, the binding of Ca²⁺ is considered very fast since preincubation of calcium with either PQQ or apo-sGDH does not influence or change the experimental kinetic traces. Considering the relative small hydrodynamic size of the calcium ion and the broad accessibility of the apo-sGDH binding pocket (the binding site of PQQ is located at the top of the barrel in a deep, broad, and positively charged cleft⁴⁰), we can legitimately assume that the second-order binding rate of Ca²⁺ to apo-sGDH is diffusion-controlled, which therefore means a rate constant k_1,Ca in the range of 10⁸–10⁹ M^–1 s^–1.⁴⁷ The kinetics of the reverse reaction can be set from the knowledge of the equilibrium dissociation constant. The latter can be estimated from the change in the absorbance of the fast pathway relative to that of the slow pathway as a function of [Ca²⁺] (Figure S3), a ratio that sets the fast equilibrium between the two pathways (slow and fast). From the analysis of this ratio, an equilibrium constant in the millimolar range was found (i.e., K_d^apo-sGDH/Ca = 5.3 mM). It follows that k_–1,Ca is in the range of 10⁵–10⁶ s^–1. This agrees with the fact Ca²⁺ is coordinated only by the two carbonyl oxygens of P248 and G247. The fast binding of PQQ to apo-sGDH/Ca²⁺ was determined from analysis of the observed rate of the fast kinetic phase as a function of [PQQ]. A bimolecular rate constant of k_1,PQQ = 1.1 × 10⁶ M^–1 s^–1 was obtained from the linear regression fit of k_1,obs as a function of [PQQ] (Figure 2), which is a rather fast rate, not far from that of a diffusion-controlled reaction. The dissociation rate of PQQ from holo_ox was then indirectly recovered from the equilibrium dissociation constant between PQQ and apo-sGDH/Ca²⁺ (K_d) that we had previously determined at room temperature in the presence of 3 mM CaCl₂ (i.e., conditions for which most of the reconstitution goes through the fast pathway).²⁹ On account of the remarkably low value of K_d^holo_ox = (3.0 ± 1.5) × 10^–11 M previously published,²⁹ a rather slow dissociation rate of k_–1,PQQ = 3.4 × 10^–5 s^–1 can be calculated.

Let us now consider the slow pathway for which the initial binding of PQQ to apo-sGDH leads to the formation of holoX_ox, followed by the binding of Ca²⁺ to generate the holo_ox form. The existence of holoX_ox was confirmed from the UV–vis absorption spectra in Figure S7, showing a maximum at 340 nm, which is 12 nm lower than for holo_ox but 12 nm higher than for free PQQ.³⁶ This was also corroborated by the fast quenching of fluorescence after rapid mixing of apo-sGDH with PQQ in the absence of Ca²⁺ (Figure S6), confirming the fast incorporation of PQQ into the apo-sGDH binding site. Such a behavior is consistent with previous works, showing that Ca²⁺ ions in the binding site are not required for a strong binding of PQQ to the apoenzyme binding site.⁴³ The calcium ion remains, however, indispensable for the enzyme activation because in its absence holoX_ox can be only very slowly reduced into holoX_red with glucose, as attested by our kinetic experiment in Figure S8, leading to a k_red,X value of 8 × 10^–4 s^–1 (which is ∼162 500-fold slower than k_red) (see Scheme 2). The slow reconstitution pathway of sGDH is expected to predominate at low calcium concentrations. For instance, most of the reconstitution goes through the slow pathway at the lowest Ca²⁺ concentration that we have tested (i.e., 9 μM). At such a low calcium concentration, the fluorescence quenching is mainly due to the binding of PQQ to apo-sGDH. In the absence of calcium, the monoexponential decay of fluorescence as a function of [PQQ] (Figure S6) allows the extraction of the bimolecular rate constant, k_2,PQQ, characterizing the binding of PQQ to the Ca²⁺-free apo-sGDH site. A value of (0.94 ± 0.20) × 10⁶ M^–1 s^–1 was obtained, which is very close to the value of k_1,PQQ (i.e., 1.1 × 10⁶ M^–1 s^–1), demonstrating that the binding rate of PQQ is not significantly affected whether there is or not a Ca²⁺ ion present in the enzyme binding site. This, however, does not inform us of the value of the dissociation rate k_–2,PQQ, which is expected to be somewhat a little higher than k_–1,PQQ because there is no Ca²⁺ in holoX_ox to further stabilize the PQQ cofactor. However, we can reasonably hypothesize that the absence of Ca²⁺ should not decrease the affinity binding of PQQ by a factor greater than 1 or 2 orders of magnitude. Therefore, for that reason, we have assumed a k_–2,PQQ value lower than 10^–2 s^–1 (meaning a K_d^holoX_ox value > 10^–8 M).

The last rate constants that are needed to fully describe the slow pathway are those associated with the two-step binding of Ca²⁺ to holoX_ox, characterizing thus the entrance of Ca²⁺ into the holoX_ox enzyme site, followed by its slow binding at the bottom of the enzyme active site sterically constrained by the presence of the PQQ cofactor. A rough estimation of the two parameters (i.e., K_2,Ca^M and k_2,Ca) characterizing this two-step Michaelis–Menten-type reaction was obtained from the stopped-flow kinetic experiments reported in Figure S10. The value of k_2,Ca^′ (1.2 s^–1) is within the range of k_2,obs (0.2 s^–1) that we had previously determined from kinetic analysis of the slow enzyme reconstitution pathway in Figure 2. According to Scheme 2, these two rate constants can be assimilated because of addressing the same rate-limiting step in the mechanism. It is worth noting that owing to the high scattering in the data of Figure S10, the values of K_2,Ca and k_2,Ca^′ are entailed by a high degree of incertitude that does not guarantee their high reliability, an issue that may explain the observed divergence between k_2,Ca and k_2,obs. We have thus finally considered the value of k_2,obs inferred from Figure 2 as the more relevant data for characterizing k_2,Ca^′.

Once a comprehensive overview of the overall kinetic rate constants characterizing the global reaction mechanism proposed in Scheme 2 was obtained, it was interesting to see whether this mechanism, with its associated rate constants, could be used to numerically simulate and predict the experimental kinetic plots of Figure 2. For such a purpose, we have used the open-source software COPASI, which allows solving numerically kinetic models with varying levels of complexity.⁴⁸ Using the overall rate constants reported in black and green in Scheme 2 and Table 1 and adjusting the rate constants in blue, we were finally able to predict quite well by simulation the UV–visible experimental kinetic traces given in Figure 2 (see Figure 5 for a side-by-side comparison of simulated curves to the experimental ones). This good agreement between simulations and experiments was made possible thanks to the iterative adjustment of K_2,Ca^M, k_2,Ca, and K_d^PQQ/Ca to values of 0.1 mM, 0.25 s^–1, and 15 mM, respectively, and also by taking into consideration the individual absorbance of each of the different intermediate species generated along the reaction (this has been achieved using the extinction coefficients gathered in Table S5 and considering the numerically simulated time-course concentration profiles of the overall species adsorbing at 338 nm). The rather excellent agreement between the simulated kinetic and experimental curves a posteriori strongly supports the proposed mechanism.

Table 1. Rate Constants Determined (in Black) Directly from the Experiments or (in Blue) Indirectly from the Best Fitting between the Numerical Kinetic Simulations and the Experimental Kinetic Plots^d.

^aMichaelis–Menten-type reactions are characterized by two parameters: the Michaelis constant (K_i^M) and the turnover rate (k′).

^bDetermined from the best fit of the simulated kinetic traces to the experimental ones (see Figure 5 and the text for details).

^cThe absolute rate constants in green were estimated by assuming a diffusion-controlled bimolecular reaction.

^dThe other rate constants (in green) were estimated by the assumption that Ca²⁺ can bind to the enzyme according to a diffusion-controlled reaction.

Figure 5.

Comparison between (left) experimental and (right) simulated UV–vis kinetic traces (monitored at 338 nm). The experimental curves in the top and bottom are the same as in Figure 1A,C, respectively. The simulated curves were obtained from the numerical simulation of the global reaction mechanism shown in Scheme 2 and using the reported rate constants (some of these constants are those determined experimentally, while others were iteratively adjusted in such a way to get the best fit between the simulations and the experimental plots).

Finally, the slow pathway seems to be the consequence of the presence of PQQ in the enzyme binding site that then hampers, through a steric effect, the access of Ca²⁺ to its enclosed position. This difficulty to enter the site while PQQ is already bound to the protein can be better apprehended in Figure 6, wherein Ca²⁺ lies at the bottom of the active site, while PQQ is located above. This steric effect is experimentally supported by the result that we have obtained with the P248A mutant (Table S4), showing that the rate-limiting step of the slow enzyme reconstitution pathway (characterized by k_2,Ca^′) can be significantly enhanced by reducing the rigidity (or increasing the flexibility) of the enzyme binding site. In addition, comparison of the X-ray structures of apo- and holo-sGDH evidences the dynamic nature of the reconstitution process since it suggests that a local rearrangement of loops 333–346 (close to the binding pocket) can occur upon PQQ binding and correct positioning of Ca²⁺ to switch on the activity (Figure 4). It is therefore possible that modulation of the overall dynamic process through steric constraints would be at the origin of the observed kinetic differences between the two pathways.

Figure 6.

Representative views of the opened and solvent-accessible cavities in (A) apo-sGDH (5MIN) and (B) holo-sGDH (1C9U), showing the different positions of the Ca atoms and the network of water molecules in apo-sGDH and illustrating the difficulty that calcium may have in accessing its binding site when PQQ binds first to the protein. The surfaces are colored according to the hydrophobicity of the residues (the more hydrophobic residues are in red).

Conclusions

We presented here a detailed description of the reconstitution mechanism of sGDH from Acinetobacter calcoaceticus. This enzyme provides an ideal playground for the comprehension of the processes through which an enzyme recovers its full activity. It is particularly relevant for enzymes that admit two different partners for a complete activation (here, PQQ and Ca²⁺). We have notably shown in this case that the order in which each of the partners binds to the protein strongly impacts the reconstitution kinetics. This might help in the design of biotechnological applications in which the dynamic of such an activity switch is crucial for the response of the system. In addition, thanks to the detailed knowledge of both structure and reactivity, we have also demonstrated that this dynamics may be modulated through the directed mutation of the binding site.

Experimental Section

Reagents

PQQ, glucose, Tris buffer, CaCl₂, dichlorophenolindophenol (DCPIP), phenazine methosulfate (PMS), and other chemicals were purchased from Sigma and used as received.

Expression, Purification, and Reconstitution of Wild-Type and Mutant sGDHs from A. calcoaceticus

A QuickChange site-directed mutagenesis kit (Stratagene) was used for the mutations. The presence of mutations was verified by DNA sequencing at the Functional Genomic Center of Bordeaux. Wild-type apo-sGDH and P248A apo-sGDH were produced, purified, and then reconstituted with PQQ and Ca²⁺, as reported earlier.⁴⁹ Apo-sGDH and holo_ox protein concentrations were assessed spectrophotometrically (Varian Cary 100) from the absorbance at 277 nm (for the extinction coefficients, see Table S5 in the Supporting Information).³⁹ Holo_red is obtained after the addition of glucose. Enzymes activities were measured using standard protocols.⁴³ In this article, the concentration of enzymes is, unless otherwise stated, expressed per monomer (or subunit). After purification and reconstitution, apo and holo forms of the enzyme were both stored at −80 °C in a 50 mM Tris buffer (pH 7.5) containing 3 mM CaCl₂.

UV–Visible Stopped-Flow Kinetics under Single Turnover

Pre-steady-state kinetic analysis was carried out on a MOS 450 stopped-flow apparatus (Biologic), and the data were analyzed using either the Biokine software package or the Origin software. The dead time of the stopped-flow apparatus was determined to be 3 ms using standard protocols.⁵⁰ All of the experiments were performed in triplicate with three different batches of enzymes. For each experiment, on average, at least four runs were performed.

Influence of CaCl₂ Preincubated in a Syringe Containing apo-sGDH or PQQ

To check whether there was any effect of calcium, we performed three sets of experiments: (i) 10 μM PQQ in syringe 1 and 5 μM apo-sGDH subunit + 6 mM CaCl₂ in syringe 2; (ii) 10 μM PQQ + 6 mM CaCl₂ in syringe 1 to 5 μM apo-sGDH subunit in syringe 2; (iii) 10 μM PQQ + 3 mM CaCl₂ in syringe 1 to 5 μM apo-sGDH + 3 mM CaCl₂ in syringe 2. Control experiments showed that the enzyme remains dimeric upon dilution.

Kinetic Studies of the Tryptophan Fluorescence Quenching

Wavelengths of excitation and emission were determined at the equilibrium by performing fluorescence quenching with a Xe lamp in the presence of 5 μM apo-sGDH subunit in the presence or absence of 10 μM PQQ in a 50 mM Tris buffer (pH 7.5) at 10 °C. The time course of sGDH fluorescence quenching induced by the binding of PQQ was recorded on a thermostated MOS 450 Biologic stopped-flow apparatus equipped with a fluorescence detector and a XeHg lamp (at 800 V). Absorption and emission wavelengths were set at 297 and 340 nm, respectively. At such wavelengths, there was no fluorescence contribution from either the buffer or PQQ. For the kinetic experiments, the first syringe was filled with 5 μM of apo-sGDH subunits and the second one with PQQ and CaCl₂ at different concentrations ranging from 5 to 40 μM for PQQ and from 9 μM to 150 mM for CaCl₂.

Formation Rate of holo_red from holo_ox

The reduction rate of holo_ox by glucose was monitored at 338 nm with the stopped-flow apparatus operating in the absorbance mode (a XeHg lamp at 200 V) thermostated at a controlled temperature of 10 °C. One syringe was filled with 5 μM of holo_ox subunit in a 50 mM Tris buffer + 3 mM CaCl₂ (pH 7.5) and the other one with 200 μM of glucose in a 50 mM Tris buffer + 3 mM CaCl₂ (pH 7.5). The final concentrations of sGDH and glucose after mixing were divided by 2.

Formation Rate of holo_red from Wild-Type or P248A apo-sGDH

The rate of PQQ reduction by glucose was measured at 338 nm with the stopped-flow apparatus operating in the absorbance mode (a XeHg lamp at 200 V) thermostated at a controlled temperature of 10 °C. One syringe was filled with 5 μM apo-sGDH subunits and the other one with a mixture of glucose, PQQ, and CaCl₂ at concentrations ranging from 10 to 80 μM for PQQ and from 18 μM to 300 mM for CaCl₂; the concentration of glucose was fixed at 200 μM. The final concentrations of sGDH, PQQ, glucose, and CaCl₂ after mixing were divided by 2.

Structure Determination and Refinement

Crystallization

Single crystals of apo-sGDH from A. calcoaceticus were grown by the vapor diffusion method. These crystals diffract to beyond 1.76 Å and are suitable for X-ray crystallography on a home source rotating anode. The space group was determined to be triclinic P(1). One asymmetric unit contains a dimer of the apo-sGDH molecule. Preliminary screening of crystallization conditions was carried out using the Jena Bioscience Classic Kit (number 2). Crystals suitable for crystallography experiments were directly obtained by the sitting-drop method (in a 96 well-plate) with drops consisting of 0.5 μL of the protein solution (at 15 mg mL^–1) and 0.5 μL of the reservoir solution equilibrated against 70 mL of the well solution at 293 K, using a reservoir solution composed of 30% (w/v) PEG 4000, 200 mM CaCl₂, and 100 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (pH 7.5) (condition D2).

Data Collection

X-ray diffraction data of apo-sGDH were collected at the IECB X-ray facility (CNRS UMS3033, INSERM US001, University of Bordeaux) at 100 K with a 3 kW Rigaku FR-X X-ray generator equipped with a Hybrid Dectris Pilatus 200 K detector. XDS and XSCALE⁵¹ were used for data integration and scaling. Statistics of data collection are listed in Table S6.

Structure Determination and Refinement

The crystal structure of apo-sGDH was determined to have 1.76 Å resolution, with phases determined by molecular replacement (1C9U as the starting model) using PHASER.⁵² Model building was performed with Coot and illustrated with PyMOL.⁵³ Crystallographic refinement was performed with programs REFMAC⁵⁴ and BUSTER-TNT.⁵⁵ Coordinates were deposited with the PDB accession code 5MIN.

Kinetic Simulations

The open-source software COPASI 4.11 was used for numerical kinetic simulations of the reconstitution mechanism shown in Scheme 2.

Supporting Information Available

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsomega.9b04034.

• PQQ structure; absorbance spectra of holo_ox-sGDH and holo_red-sGDH; transient kinetics of the reduction of sGDH by d-glucose; effect of [CaCl₂] on ΔA₁ and ΔA₂; plots of k_1,obs and k_2,obs with [PQQ], fluorescence spectra of apo-sGDH; time course of tryptophan fluorescence quenching; absorption spectra of the different sGDH forms; kinetics of reduction of holoX_ox to holoX_red; influence of CaCl₂ on the conversion of holoX_red into holo_red and holoX_ox into holo_red; electron density map of apo-sGDH; observed rates and absorbance difference amplitudes extracted from Figures 1A,C, and 3B; k_1,obs and k_2,obs determined for the wild-type and P248A mutant of sGDH; and extinction coefficients of the species involved in the sGDH reconstitution (PDF)

Accession Codes

Accession codes: sGDH F0KFV3.

The authors declare no competing financial interest.