The following information is missing from the Data Availability statement: The plasmids described in this study are available from Addgene:
plasmid pBDC, Addgene ID: 135188
plasmid pBDK, Addgene ID: 135190
plasmid pCNA, Addgene ID: 135191
plasmid pSNA, Addgene ID: 135192
plasmid pSNK, Addgene ID: 135193
There is an error in S2 Table. The fourteenth base I-SceI should be “T,” not “A.” The correct S2 Table provided here shows the whole sequence of plasmid pSNA (Addgene ID: 135192).
Supporting information
S2 Table. Sequences of I-SceI, I-CreI, and GC cassette.
(DOCX)
Click here for additional data file. (35.7KB, docx)
Reference
- 1.Wei Y, Deng P, Mohsin A, Yang Y, Zhou H, Guo M, et al. (2017) An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome. PLoS ONE 12(10): e0186891 10.1371/journal.pone.0186891 [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
S2 Table. Sequences of I-SceI, I-CreI, and GC cassette.
(DOCX)
Click here for additional data file. (35.7KB, docx)