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. 2009 Dec 30;25(4):225–230. doi: 10.5487/TR.2009.25.4.225

Micronucleus Test of DHU001, a Polyherbal Formula, in Bone Marrow Cells of Male ICR Mice

Seong-Soo Roh 19, Hyeung-Sik Lee 29, Sae-Kwang Ku 39,✉
PMCID: PMC7006286  PMID: 32038842

Abstract

The genotoxic effects of DHU001, a polyherbal formula were evaluated using the mouse micronucleus test. DHU001 was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000 and 500 mg/kg. Cyclophosphamide was used as a known geno-toxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. In addition, the changes on the total white blood cells and differential counts on the lymphocytes, neutrophils, eosinophils, basophils and monocytes in the prepared blood smears were also conducted to observe the possible immunosuppression. The results indicats that DHU001 showed no genotoxicity effects up to 2000 mg/kg dosing levels and did not influenced on the total white blood cells and differential counts. In addition, it is also considered that there were no problems from cytotoxicity of DHU001 tested in this study because the polychromatic erythrocyte ratio was detected as > 0.41 in all tested groups.

Key words: DHU001, Micronucleus test, Genotoxicity, Mice, White blood cells

References

  1. Aimbire F, Penna S, Rodrigues M, Rodrigues KC, Lopes-Martins RA, Sertié JA. Effect of hydroalcoholic extract of Zingiber officinalis rhizomes on LPS-induced rat airway hyperreactivity and lung inflammation. Prostaglandins Leukot Essent Fatty Acids. 2007;77:129–138. doi: 10.1016/j.plefa.2007.08.008. [DOI] [PubMed] [Google Scholar]
  2. Ashby J. Is there a continuing role for the intraperito-neal injection route of exposure in short-term rodent genotoxicity assays? Mutat. Res. 1985;156:239–243. doi: 10.1016/0165-1218(85)90069-2. [DOI] [PubMed] [Google Scholar]
  3. Back YD, Lee HS, Ku SK. Effects of DHU001, a mixed herbal formula on acute inflammation in mice. Toxicol. Res. 2008;24:189–194. doi: 10.5487/TR.2008.24.3.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dourish, C.T (1987). Effects of drugs on spontaneous motor activity in experimental psychopharmacology (Greenshaw, A.J and Dourish, C.T, Eds.), Humana Press, Clifton, pp. 325–334.
  5. El-Bayoumy K. The protective role of selenium on genetic damage and on cancer. Mutat. Res. 2001;475:123–139. doi: 10.1016/S0027-5107(01)00075-6. [DOI] [PubMed] [Google Scholar]
  6. Ghayur MN, Gilani AH, Janssen LJ. Ginger attenuates acetylcholine-induced contraction and Ca2+ signalling in murine airway smooth muscle cells. Can. J. Physiol. Pharmacol. 2008;86:264–271. doi: 10.1139/Y08-030. [DOI] [PubMed] [Google Scholar]
  7. Grochow, L.B (1996). Covalent-DNA binding drugs in The chemotherapy source book (Perry, M.C., Ed.), Williams & Wilkins, Baltimore, pp. 293–316.
  8. Heddle JA. A rapid in vivo test for chromosome damage. Mutat. Res. 1973;18:187–190. doi: 10.1016/0027-5107(73)90035-3. [DOI] [PubMed] [Google Scholar]
  9. Heddle J, Hite M, Kirkhart B, Mavournin K, MacGregor JT, Newell GW, Salamone MF. The induction of micronuclei as a measure of genotoxicity: a report of the U.S. environmental protection agency gene-tox program. Mutat. Res. 1983;123:61–118. doi: 10.1016/0165-1110(83)90047-7. [DOI] [PubMed] [Google Scholar]
  10. Heddle, J.A, Stuart, E. and Salamone, M.F (1984). The bone marrow micronucleus test in Handbook of mutagenicity test procedures (Kilbey, B., Legator, M., Nichols, W. and Ramel, C., Eds.), Elsevier, Amsterdam, pp. 441–457.
  11. Kalantari H, Larki A, Latifi SM. The genotoxic-ity study of garlic and pasipy herbal drops by peripheral blood micronucleus test. Acta Physiol. Hung. 2007;94:261–266. doi: 10.1556/APhysiol.94.2007.3.10. [DOI] [PubMed] [Google Scholar]
  12. Kim SS, Lee SC, Shin HD, Shin MK, Kim JH, Song HJ. Studies on allergy asthma effect of Radix Platicodi. Kor. J. Herbology. 2004;19:61–70. [Google Scholar]
  13. Korea FoodDrug Administration. Testing Guidelines for Safety Evaluation of Drugs. 2005. [Google Scholar]
  14. Lee JE, Kim HJ, Choi EK, Chai HY, Yun YW, Kim DJ, Nam SY, Lee BJ, Ahn BW, Kang HG, Kim YB. Four-week repeated-dose toxicity study on Pinellia Extract. Korean J. Lab. Anim. Sci. 2003;19:127–141. [Google Scholar]
  15. Matter B, Schmid W. Trenimon-induced chromosomal damage in bone-marrow cells of six mammalian species, evaluated by the micronucleus test. Mutat. Res. 1971;12:417–425. doi: 10.1016/0027-5107(71)90092-3. [DOI] [PubMed] [Google Scholar]
  16. Miyauchi A, Hiramine C, Tanaka S, Hojo K. Differential effects of a single dose of cyclophosphamide on T cell subsets of the thymus and spleen in mice: flow cytofluorometry analysis. Tohoku J. Exp. Med. 1990;162:147–167. doi: 10.1620/tjem.162.147. [DOI] [PubMed] [Google Scholar]
  17. Narimanian M, Badalyan M, Panosyan V, Gabrielyan E, Panossian A, Wikman G, Wagner H. Impact of Chisan (ADAPT-232) on the quality-of-life and its efficacy as an adjuvant in the treatment of acute nonspecific pneumonia. Phytomedicine. 2005;12:723–729. doi: 10.1016/j.phymed.2004.11.004. [DOI] [PubMed] [Google Scholar]
  18. Organization for Economic Co-OperationDevelopment., editor. Guideline for the Testing of Chemicals TG No. 474. Mammalian Erythrocyte Micronucleus Test. 1997. [Google Scholar]
  19. Irwin S. Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse. Psychopharmacology. 1968;13:222–257. doi: 10.1007/BF00401402. [DOI] [PubMed] [Google Scholar]
  20. Park JH, Geon DG. Pharmacognostical studies on the Chinese crude drug “Maig Moon Dong”. Kor. J. Pharmacogn. 2003;34:6–9. [Google Scholar]
  21. Renner HW. In vivo effects of single or combined dietary antimutagens on mutagen-induced chromosomal aberrations. Mutat. Res. 1990;244:185–188. doi: 10.1016/0165-7992(90)90070-Z. [DOI] [PubMed] [Google Scholar]
  22. Rhyu MR, Kim EY, Yoon BK, Lee YJ, Chen SN. Aqueous extract of Schizandra chinensis fruit causes endothelium-dependent and -independent relaxation of isolated rat thoracic aorta. Phytomedicine. 2006;13:651–657. doi: 10.1016/j.phymed.2006.02.003. [DOI] [PubMed] [Google Scholar]
  23. Schimid W. The Micronucleus Test. Mutat. Res. 1975;31:9–15. doi: 10.1016/0165-1161(75)90058-8. [DOI] [PubMed] [Google Scholar]
  24. Shin TY. Inhibition of immunologic and nonimmuno-logic stimulation-mediated anaphylactic reactions by the aqueous extract of Mentha arvensis. Immunopharmacol. Immunotoxicol. 2003;25:273–283. doi: 10.1081/IPH-120020475. [DOI] [PubMed] [Google Scholar]
  25. Sun HX, Pan HJ. Immunological adjuvant effect of Glycyrrhiza uralensis saponins on the immune responses to ovalbumin in mice. Vaccine. 2006;24:1914–1920. doi: 10.1016/j.vaccine.2005.10.040. [DOI] [PubMed] [Google Scholar]
  26. Von Ledebur M, Schmid W. The micronucleus test - methodological aspects. Mutat. Res. 1973;19:109–117. doi: 10.1016/0027-5107(73)90118-8. [DOI] [PubMed] [Google Scholar]

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