Figure 2: MART1 TCR⁺ cells are enriched in an A2⁺ compared to an A2⁻ or Swine thymus 10–14 weeks post-transplant.

Representative FCM staining of thymus grafts at takedown of transduced cells developing in a a. human HLA-A2⁺, b. swine or c. human HLA-A2⁻ thymus. Proportion of d. GFP⁺ cells and e. GFP⁺ MART1 TCR⁺ cells in the thymus grafts of recipient mice (A2⁺ n = 6, Sw n = 6, A2⁻ n=4; one A2⁻ mouse was excluded from tetramer analysis due to too few detected GFP⁺ events). f., g. Example control staining of thymus grafts of animals transplanted with f. CD34⁺ cells transduced with a TCR^null GFP-only lentivirus and with g. untransduced CD34⁺ cells. * = p < 0.05% by Mann-Whitney U test.