. Author manuscript; available in PMC: 2020 Nov 27.

Published in final edited form as: Cell. 2019 Nov 6;179(6):1319–1329.e8. doi: 10.1016/j.cell.2019.10.036

Table 1.

Summary of cryo-EM data collection, 3D reconstruction, and model refinement

	FLCN-FNIP2-Rag-Ragulator
Imaging parameters and 3D reconstruction
Cryo-EM grids	Quantfoil R 1.2/1.3 Cu 400
Calibrated magnification	48,077
Acceleration voltage [kV]	300
Pixel size [Å]	1.04
Total dose [e⁻/Å²]	59.2
Exposure time [s]	6.4
Defocus range [μm]	−1.0 to −3.5
Particles in final 3D reconstruction	126,984
Resolution (‘Gold-standard’ at FSC 0.143) [Å]	3.31
Model refinement
Resolution in phenix.real_space_refine [Å]	3.6
No. atoms: protein	16353
Ligands / ions	GppNHp, GDP, Mg²⁺
r.m.s. deviations: bond lengths [Å]	0.006
r.m.s. deviations: bond angles [°]	0.908
MolProbity score	1.78
EMRinger score	2.02
Rotamer outliers [%]	0.61
Ramachandran angles: favored [%]	91.5
Ramachandran angles: allowed [%]	8.40
Ramachandran angles: outliers [%]	0.10
PDB	6ULG
EMDB	EMD-20814