In the article, “Deletion of murine choline dehydrogenase results in diminished sperm motility” by Amy R. Johnson, Corneliu N. Craciunescu, Zhong Guo, Ya-Wen Teng, Randy J. Thresher, Jan K. Blusztajn, and Steven H. Zeisel, in FASEB J. August 2010 24:2752–2761 (doi: 10.1096/fj.09-153718), there is an error that the authors wish to correct.
Figure 1 in the article, illustrating the targeting, the KO allele, and the position of the primers, was incorrect when it showed that exons 1–3 in the Chdh gene were deleted. The authors recently sequenced the region of chromosome 14 that codes for the Chdh gene in the knockout mouse (30009023–30040466 bp, + strand), and they discovered that exons 1 through 6 and much of exon 7, which contains the 3′UTR, were deleted (and not, as was suggested in the article, that only exons 1–3 were deleted). Thus, the common primer is in the 3′UTR region of exon 7 (not upstream of exon 1), and the wild-type primer is in exon 6 (not in intron 3). Fig. 1 below presents a snapshot of the alignment data for the region around the gene, which illustrates the deleted exons in Chdh gene region. As stated in the original publication in 2010, the Chdh-knockout mouse does not make a functional gene product. This correction does not change the conclusions in the article.
Figure 1.
Sequencing of the Chdh-knockout mouse shows that exons 1–6 and part of exon 7 are deleted (the 3′UTR region of exon 7 is intact). A genomic DNA sample from a Chdh-knockout mouse was sequenced at the University of North Carolina at Chapel Hill’s High Throughput Sequencing Facility. The sample was run on the Illumina HiSeq 4000 High Output as paired-end 150× with a loading concentration of 2.5 nM.
DOI: 10.1096/fj.09-153718ERR
