Figure 3.

Production of interleukin 8 by neutrophils is impaired during ibrutinib therapy. (A) Neutrophils from whole blood taken from patients before ibrutinib treatment, and 1 and 3 months after starting ibrutinib therapy were untreated (exposed to phosphate-buffered saline, PBS), or stimulated with Aspergillus germinating conidia or lipopolysaccharide for 4 h at 37°C. Brefeldin A was added after 30 min of stimulation. Cells were stained with membrane antibodies, permeabilized, stained with interleukin 8 (IL-8) antibody and then analyzed by flow cytometry. The results are expressed as the percentage of positive cells. (B) Whole blood samples were stimulated with Aspergillus germinating conidia for 2 h. The supernatants were collected and tested for IL8 concentration.