Figure 2.

3F_ax-Neu5Ac treatment decreases sialylation in the MM1S^Heca452 cell line. MM1S^Heca452 cells were treated with 300 mM 3F_ax-Neu5Ac or dimethyl sulfoxide (DMSO) (vehicle control) for seven days. After treatment, cells were collected and stained with the Heca452 (A and B), CD15s (C and D), MALII (F and G), or SNA (G and H) antibodies or lectins. Bars represent mean±Standard Error of Mean of three independent experiments. Unpaired Student’s t-test was used to determine statistical significance. *P<0.05; ** P<0.01; ***P<0.001. MFI: median fluorescence intensity.