Figure 5.

3F_ax-Neu5Ac treatment reduces motility of MM1S^Heca452 independently of SDF1α. MM1S^Heca452 cells were treated with 300 mM 3F_ax-Neu5Ac or dimethyl sulfoxide (DMSO) (vehicle control) for seven days. After treatment, cells were starved for 1 hour (h) and then seeded on the upper chamber of transwells. Lower chamber was filled with either serum-free media (No SDF1α) or serum-free media supplemented with SDF1α (20 ng/mL). Cells were allowed to migrate for 4 h at 37°C. After incubation, cells in the lower chambers were collected and counted using a BD Accuri flow cytometer. Data are presented as (A) raw data or (B) as the difference between migrating cells in SDF1α-containing media and control media. Bars represents mean±Standard Error of Mean of three independent experiments. One-way ANOVA test was used to determine statistical significance with Sidak’s multiple comparison post-hoc testing. *P<0.05; **P<0.01; ***P<0.001; ns: non-significant.