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TDP-43 overexpression increases TDP-43 protein full-length (43kDa) presence in soluble and insoluble fractions of cells lysates (B) together with the detection of 35- (C) and 25-kDa (D) C-terminal fragments (CTFs). Representative Western blots are shown in panel (A). CE: crude extract; S: soluble fraction; P: insoluble fraction. Nonparametric Kruskal–Wallis test revealed * p < 0.05 and # p = 0.053 compared to the respective control groups (CE x CE; S x S; P x P; n = 3–6).
