Table 1.

PTMs of different members of TRPC as reported by traditional experimental studies.

Channel	Modification	Species and Accession Number	Site	Sequence	Enzyme	Cell	Effect	Reference
TRPC1	Phosphorylation	Human n.a.	S/T/Y	n.a.	PKCα	HUVEC and HMEC	Increased TRPC1 activity, enhanced permeability of endothelial monolayers	[47]
TRPC1	Phosphorylation	Rabbit n.a.	S/T	n.a.	PKC	Rabbit portal vein myocytes	Increased TRPC1 activity, promoted PIP2-mediated SOCE	[51]
TRPC1	Phosphorylation	Human P48995.2	S172 T313	SAKNKKDSLRHSRFR SGYRRKPTCKKIMTV	PKG	Porcine coronary arterial smooth muscle cell	Decreased TRPC1 activity, inhibited NO-, PKG-induced smooth muscle hyperpolarization and relaxation	[55]
TRPC1	Phosphorylation	Human	S/T/Y	n.a.	PKCα	HUVEC	Increased TRPC1 activity, promoted monocyte binding to endothelial cells and increased endothelial hyperpermeability	[48]
TRPC1	Phosphorylation	Rabbit n.a.	S/T	n.a.	PKC	Rabbit vascular smooth muscle cell	Increased TRPC1 activity	[52]
TRPC1	Phosphorylation	Human P48995.2	S172 T313	SAKNKKDSLRHSRFR SGYRRKPTCKKIMTV	PKG	Primary mesenteric artery endothelial cells	Decreased TRPC1 activity	[56]
TRPC1	Phosphorylation	Rat	S/T	n.a.	PKCα	INS-1E	Increased TRPC1 activity, promoted insulin secretion	[54]
TRPC3	N-glycosylation	Human Q13507.3	N416 (N418 *)	EGITTLPNITVTDYP	n.a.	COS-M6 and HEK-293	n.a.	[58,59]
TRPC3	Phosphorylation	Human Q13507.3	S712	PPFSLVPSPKSFVYF	PKC	HEK-293	Decreased TRPC3 activity	[63]
TRPC3	Phosphorylation	Human Q13507.3	T11 S263	SPSLRRMTVMREKGR KNDYRKLSMQCKDFV	PKG	HEK-293	Decreased TRPC3 activity, abolished the SOCE mediated by TRPC3	[62,64]
TRPC3	Phosphorylation	Human Q13507.3	Y49 Y148 Y150 Y226	RFLDAAEYGNIPVVR ELQDDDFYAYDEDGT QDDDFYAYDEDGTRF KGLASPAYLSLSSED	Src	HEK	Phosphorylation at Y226 but not others increased TRPC3 activity	[61]
TRPC3	Phosphorylation	Human Q13507.3	T573	LQISLGRTVKDIFKF	PKC	HL-1	Coupled TRPC3 signaling to the activation of the NFAT pathway	[65]
TRPC4	Phosphorylation	Human Q9UBN4.1	Y959 Y972	EEDSSIDYDLNLPDT DTVTHEDYVTTRL	Src	COS-7	Induced insertion of TRPC4 into plasma membrane and its association with NHERF	[70]
TRPC4	ubiquitination	Mouse Q9QUQ5.2	N terminus	n.a.	AIP4	HEK-293T	Promoted endocytosis of TRPC4 and reduced its basal activity	[75]
TRPC4	Phosphorylation	Mouse Q9QUQ5.2	S688	KMRRKPESFGTIGRR	PKG	HEK-293	Increased TRPC4 activity	[71]
TRPC4	Disulfide bond	Mouse Q9QUQ5.2	C549 C554	TKGLSCKGIRCEKQNN	n.a.	n.a.	Constrained TRPC4 activity	[76]
TRPC5	Phosphorylation	Mouse Q9QX29.1	T972	DGQEEQVTTRL	PKC	HEK-293	Accelerated desensitization of TRPC5, necessary for the association with NHERF	[36,77]
TRPC5	S-nitrosylation	Mouse Q9QX29.1	C553 C558	DEPNNCKGIRCEKQNN	n.a.	HEK, vascular endothelial cells	Increased TRPC5 activity	[82]
TRPC5	Disulfide bond	Human Q9UL62.1	C553 C558	DEPNNCKGIRCEKQNN	n.a.	HEK-293	Constrained TRPC5 activity	[79]
TRPC5	Phosphorylation	Human Q9UL62.1	S794 S796	SGGARAKSKSVSFNL	PKA	HEK-293	Decreased TRPC5 activity	[78]
TRPC5	S-glutathionylation	Human Q9UL62.1	C176 C178	RPHQIRCNCVECVSS	n.a.	HEK-293, Clonal striatal cells	Increased TRPC5 activity, activated the calmodulin- dependent protein kinase and calpain-caspase pathway	[87]
TRPC5	Disulfide bond	Human Q9UL62.1	C553 C558	DEPNNCKGIRCEKQNN	n.a.	HEK-293	Stabilized TRPC5 multimerization, promoted its plasma membrane insertion	[80]
TRPC5	Disulfide bond	Mouse Q9QX29.1	C553 C558	DEPNNCKGIRCEKQNN	n.a.	n.a.	Essential for TRPC5 activation	[81]
TRPC6	N-glycosylation	Mouse Q61143.1	N472 N560	EGTKLLPNETSTDNA AQSIIDANDTLKDLT	n.a.	COS	n.a.	[6]
TRPC6	N-glycosylation	Rat Q99N78.1	N711	YVLYGVYNVTMVIVL	n.a.	COS	n.a.	[88]
TRPC6	N-glycosylation	Human Q9Y210.1	N473 N561	EGTKLLPNETSTDNA AQSIIDANDTLKDLT	n.a.	HEK-293	Decreased TRPC6 constitutive activity	[59]
TRPC6	Phosphorylation	n.a.	Y	n.a.	src	COS-7	Increased TRPC6 activity	[96]
TRPC6	Phosphorylation	Rat Q99N78.1	S768	VPFNLVPSPKSLLYL	PKC	PC12D	Decreased TRPC6 activity, required for protein complex formation upon activation of the M1 muscarinic acetylcholine receptor	[113]
TRPC6	Phosphorylation	Mouse Q61143.1	T69	RLTHRRQTILREKGR	PKG	HEK-293 and A7r5	Decreased TRPC6 activity	[86]
TRPC6	Phosphorylation	Mouse Q61143.1	S448	KFVAHAASFTIFLGL	PKC	HEK-293T and A7r5	Decreased TRPC6 activity, reduced vasopressin-induced Ca2+ entry	[107]
TRPC6	Phosphorylation	Mouse Q61143.1	T69	RLTHRRQTILREKGR	PKG	NRVM	Decreased TRPC6 activity, suppressed NFAT activation, prevented cardiac hypertrophy	[100,102]
TRPC6	Phosphorylation	Human Q9Y210.1	T70 S322	RLAHRRQTVLREKGR KNDYKKLSMQCKDFV	PKG	NRVM and AMVM	Decrease TRPC6 activity, suppressed NFAT activation, prevented cardiac hypertrophy	[101]
TRPC6	Phosphorylation	Mouse Q61143.1	T69	RLTHRRQTILREKGR	PKA	RAoSMC	Decreased TRPC6 activity, attenuated angiotensin II-induced vasoconstriction	[104]
TRPC6	Phosphorylation	Mouse Q61143.1	S814	KKFGISGSHEDLSKF	n.a., but not casein kinase II	HEK-293	No effect on TRPC6 activity	[112]
TRPC6	Phosphorylation	Mouse Q61143.1	S281	NAYKGLASPAYLSLS	ERK1/2	HEK-293 and Rat Glomerular mesangial cell	Increased TRPC6 activity, necessary for cAMP-induced Ca2+ influx	[98]
TRPC6	Phosphorylation	Mouse Q61143.1	Y284	KGLASPAYLSLSSED	Src	HEK-293T, podocyte	Enhanced plasma membrane trafficking of TRPC6	[109]
TRPC6	Phosphorylation	Mouse Q61143.1	S28 T69	AGTRRNESQDYLLMD RLTHRRQTILREKGR	PKA	HEK-293	S28 but not T69 decreased TRPC6 activity	[105]
TRPC6	Phosphorylation	Mouse Q61143.1	T69	RLTHRRQTILREKGR	cGKI	MLEC and HDMEC	Decreased TRPC6 activity, prevented endothelial cell hyperpermeability responses to hyperforin	[106]
TRPC6	Phosphorylation	Mouse Q61143.1	T487	RQLFRMKTSCFSWME	CaMKII	HEK293 and A7r5	Increased TRPC6 activity, enhanced arginine vasopressin-induced cation current in smooth muscle cells	[97]
TRPC6	Phosphorylation	Mouse Q61143.1	T69 S321	RLTHRRQTILREKGR KNDYKKLSMQCKDFV	PKG	Murine podocyte	Decreased TRPC6 activity, reduced podocyte motility	[108]
TRPC6	Phosphorylation	Dog	T	n.a.	n.a.	Canine heart left ventricle	Decreased TRPC6 activity, underpinned the tadalafil-caused delay of dystrophic cardiomyopathy onset	[103]
TRPC6	Phosphorylation	Human Q9Y210.1	S4 S13, S14 S814	MSQSPAFGPRR AFGPRRGSSPRGAAG KKLGILGSHEDLSKL	MAPK Cdk-5	HEK-293T	S14 enhanced TRPC6 plasma membrane trafficking and constitutive activity	[110]
TRPC6	N-glycosylation	Human Q9Y210.1	N473 N561	EGTKLLPNETSTDNA AQSIIDANDTLKDLT	n.a.	TRex293, podocyte	Required for channel activity, ERK activation and cytotoxicity of the GOF TRPC6 mutant (R895C)	[89]
TRPC7	Phosphorylation	Mouse Q9WVC5.1	T15	KNMQRRHTTLREKGR	cGKI	COS-7 and HEK-293T	Decreased TRPC7 activity	[115]
TRPC7	Phosphorylation	Mouse Q9WVC5.1	S714	APFNLVPSPKSFYYL	PKC	MEF	Decreased TRPC7 activity, regulated cytoskeleton organization and myofibroblast phenotype	[116]

* Denotes PTM sites reported in the original papers but they are actually the same sites as indicated in the table. INS-1E: rat insulinoma cells. HUVEC: human umbilical vein endothelial cells. HMEC: human microvessel endothelial cells. COS: African green monkey kidney fibroblast-like cell line. HEK: human embryonic kidney cell line. HL-1: murine atrial myocytes. Hela: human cervix epithelia cell line. PC12D: rat adrenal gland cell line. NRVM: neonatal rat ventricular myocyte. AMVM: adult mouse ventricular myocyte. RAoSMC: rat aortic smooth muscle cell. MLEC: mouse microvascular lung endothelial cell. HDMEC: human dermal microvascular endothelial cell. Cdk-5: cyclin-dependent kinase 5. MEF: murine embryonic fibroblasts. A7r5: vascular smooth muscle cell line.