Table 1.
Blockers used.
| Target Group | Blocker | Target | Solvent | Conc. (µM) | Manufacturer | Catalogue Number |
|---|---|---|---|---|---|---|
| Glutamatergic | MPEP | mGluR5 | DMSO | 25 | Tocris | 1212 |
| TFB-TBOA | EAATs | DMSO | 1 | Tocris | 2532 | |
| APV | NMDA | NaHCO3 | 100 | Cayman Chem. | 14,540 | |
| CNQX | AMPA | DMSO | 25 | Cayman Chem. | 14,618 | |
|
GABAergic/
Glycinergic |
Bicuculline | GABAA | σ H2O | 10 | Sigma-Aldrich | 14,343 |
| CGP-55845 | GABAB | σ H2O | 5 | Sigma-Aldrich | SML0594 | |
| NNC-711 | GAT1 | DMSO | 100 | Tocris | 1779 | |
| SNAP-5114 | GAT2/3 | DMSO | 100 | Sigma-Aldrich | S1069 | |
| NPA | GAT1/2/3 | σ H2O | 100 | Tocris | 0236 | |
| Sarcosine | GlyTs | σ H2O | 500 | Sigma-Aldrich | 131,776 | |
| Cholinergic | α-Bungarotoxin | α7 nAChR | σ H2O | 0.1 | Sigma-Aldrich | T0195 |
| Atropine | mAChR | σ H2O | 5 | Sigma-Aldrich | A0132 | |
| Purinergic | PPADs | P2X/Y | σ H2O | 20 | Sigma-Aldrich | P178 |
| Caffeine | P1 | σ H2O | 100 | Sigma-Aldrich | C0750 | |
| Adrenergic | Prazosin | α1 receptor | σ H2O | 0.2 | Sigma-Aldrich | P7791 |
| Propranolol | β receptor | σ H2O | 10 | Sigma-Aldrich | P0084 | |
| Na+ dependant transporters | TTX | Nav | σ H2O | 0.5 | BioTrend | BN0518 |
| SEA0400 | NCX | DMSO | 10 | MCE | HY15515 | |
| Bumetanide | NKCC1 | DMSO | 40 | BioTrend | BG0113 | |
| pH | Amiloride | NHE | σ H2O | 50 | Sigma-Aldrich | BP008 |
| Hemichannels | La3+ | TRP/Hemicha. | σ H2O | 50 | Merck | 203,521 |
Abbreviations are as follows: MPEP (2-Methyl-6-(phenylethynyl)pyridine), TFB-TBOA ((3S)-3-[[3-[[4-(Trifluoromethyl)benzoyl]amino]phenyl]methoxy]-l-aspartic acid), APV ((2R)-amino-5-phos-phonovaleric acid; (2R)-amino-5-phosphonopentanoate), CNQX (6-cyano-7-nitroquinoxaline-2,3-dione), CPG-55845 ((2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydro xypropyl] (phenyl-methyl)phosphinic acid hydrochloride), NNC-711 (1,2,5,6-tetrahydro-1-[2-[[(diphenylmethylene) amino]oxy]ethyl]-3-pyridinecarboxylic acid hydrochloride), SNAP-5114 (1-[2-[tris(4-methoxy-phenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid), NPA (nipecotic acid), PPADs (pyridoxal-phosphate-6-azophenyl-2’,4’-disulfonic acid), TTX (tetrodotoxin), SEA0400 (2-[4-[(2,5-difluoro-phenyl)methoxy]phenoxy]-5-ethoxyaniline), mGluR5 (metabotropic glutamate receptor 5), EAATs (excitatory amino acid transporters), NMDA (N-methyl-d-aspartate receptors), AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor), GAT (gamma-aminobutyric acid trans-porters), GlyT (glycine transporters), nACh (nicotinic acetylcholine receptors), mACh (muscarinic acetylcholine receptors), Nav (voltage gated sodium channels), NCX (sodium calcium exchanger), NKCC1 (sodium potassium chloride co-transporter), NHE (sodium proton exchanger), TRP (transient receptor potential channels). Manufacturers were located as follows (third party distributers are indicated in bold): Merck KGaA, Darmstadt, Germany; Biotrend, Köln, Germany; MCE, Hycultec, Beutelsbach, Germany; Tocris, Bio-Techne GmbH, Wiesbaden Germany; A.G. Scientific, Mobitec, Göttingen Germany; Cayman chemical, Biomol GmbH, Hamburg Germany; Sigma-Aldrich Chemie GmbH, Munich, Germany.