Table 2.
Primer sequences for qPCR gene expression analysis.
| Sequence | ||
|---|---|---|
| Gene | 5′-Sense-3′ | 5′-Anti-Sense-3′ |
| Gapdh | CGACTTCAACAGCAACTCCCACTCTTCC | TGGGTGGTCCAGGGTTTCTTACTCCTT |
| Mcl-1 | GGAAGTCCTCGCCTGCGTCA | AAACATGGTCGGACGCCGCA |
| Bcl-xL | AGGCAGGCGATGAGTTTGAA | CGGCTCTCGGCTGCTGCATT |
| Hax-1 | GACCTTGCCTTCCCACTCTCCTGA | GTCCCTGCGACCCCCAATCTG |
| OCT4 pair1 * | GTGAGCCGTCTTTCCACCAGG | GGGTGAGAAGGCGAAGTCTG |
| OCT4 pair2 # | CCCTCCCTGGGGATGCTGTGAG | GAGTGACAGACAGGCCAGGCTCC |
| Nanog | TGCGGCTCACTTCCTTCTGACTTC | GGCCCTTGTCAGCCTCAGGAC |
| TCTP | TATATGAGGTTGGGGAGCGCCCG | CCTCCGGACCTTCAGCGGAA |
* The pair of primers are located on exon 1 of OCT4 gene. # The pair of primers are located on exon 4 of OCT4 gene.