Table 6. Mutation Spectra With and Without DinB.
| Mutation type | Lee et al. 2012 E. coli mutation accumulation unselected | Wagner and Nohmi, 2000 (phage lambda C gene) | Unselected mutations associated with: | |||
|---|---|---|---|---|---|---|
| Spontaneous | Overproduced DinB | 32a Light-moderate stables | 7b Heavy stables | 20c Light unstables | ||
| Substitutions (total) | 233 (91%) | 55 (59%) | 30 (33%) | 46 (85%) | 189 (67%) | 13 (93%) |
| Transitions | 131 (51%) | 6 (6%) | 12 (13%) | 12 (22%) | 63 (22%) | 4 (29%) |
| A/T->G/C | 49 | 2 | 9 | 5 | 35 | 1 |
| G/C->A/T | 82 | 4 | 3 | 7 | 28 | 4 |
| Transversions | 102 (40%) | 49 (53%) | 18 (20%) | 34 (63%) | 126 (44%) | 9 (64%) |
| A/T->T/A | 17 | 2 | 0 | 5 | 2 | 2 |
| 38 | 0 | 7 | 14 | 38 | 4 | |
| G/C->T/A | 30 | 47 | 6 | 8 | 54 | 4 |
| G/C->C/G | 17 | 0 | 5 | 7 | 32 | 5 |
| Indels (total) | 21 (8%) | 37 (40%) | 59 (66%) | 8 (15%) | 95 (33%) | 1(7%) |
| −1 one base | 13 | 7 | 54 | 6 | 93 | 0 |
| +1 one base | 6 | 28 | 3 | 1 | 0 | 0 |
| indel > 1 base | 2 | 2 | 2 | 1 | 2 | 1 |
| Overall total | 254 | 92 | 89 | 54 | 284 | 14 |
a
Stable revertants (32) with light or moderate mutagenesis (<5 mutations): S1, S2, S3, S4, S5, S7, S9, S10, S11, S12, S13, S14, S15, S16, S17, S18, S19, S20, S21, S22, S24, S25, S27, S28, S29, S31, S32, S33, S34, A57, A59, A61.
b
Stable revertants (7) with heavy mutagenesis (≥10 mutations): S6, S23, S26, S30, A58, A60, A62.
c
Unstable revertants (20) with light or moderate mutagenesis (<10 mutations): U0, U1, U2, U3, U4, U5, U6, U7, U8, U9, U10, U11, U12, U13, U14, U15, U16, U17, U18, U19 (There were no heavily mutagenized unstables).