. 2019 Dec 18;9(1):11. doi: 10.3390/cells9010011

Table 5.

Intracellular TNFα and IFN-γ staining in CD4+ T cells and macrophages after CIA.

Day 4	WT vs. IL-18Rα KO
Day 4	TNFα	IFN-γ
CD4⁺	3.8 ± 0.5 vs. 4.4 ± 0.6	1.4 ± 0.1 vs. 0.9 ± 0.1 **
F4/80⁺	2.3 ± 0.6 vs. 0.9 ± 0.1 *	1.5 ± 0.4 vs. 0.6 ± 0.1 *
CD11b⁺	3.0 ± 0.7 vs. 1.3 ± 0.1 *	2.1 ± 0.5 vs. 0.8 ± 0.1 *
F4/80⁺CD11b⁺	1.2 ± 0.7 vs. 0.5 ± 0.1 *	0.8 ± 0.5 vs. 0.3 ± 0.1 *

The percentages of TNFα⁺ and IFN-γ⁺ in CD4⁺ T cells, F4/80⁺, CD11b⁺, and F4/80⁺CD11b⁺ cells were measured by FACS analysis. The data are mean ± SEM. * p < 0.05 and ** p < 0.01, WT (n = 10) vs. IL-18Rα KO mice (n = 15). CIA: collagen-induced arthritis; IFN-γ: interferon-gamma; IL-18Rα KO: interleukin-18 receptor-alpha knock-out; LPS: lipopolysaccharide; TNF: tumor necrosis factor; WT: wild-type.