Western blot analysis of Sirtuin 1 (SIRT1) (a), SIRT3 (b), peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1α) (c), mitochondrial transcriptional factor A (mtTFA) (d) and superoxide dismutase 2 (SOD2) (e) and representative images of immunoreactive bands (f). Data are presented as means ± SEM of densitometric analysis of immunoreactive bands normalized to internal reference protein (glyceraldehyde-3-phosphate dehydrogenase, GAPDH). Three mice per experimental group were employed. Experiments were done in triplicate. *, p < 0.05, **, p < 0.01, ***, p < 0.001, t-test.