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. 2020 Jan 6;9(1):132. doi: 10.3390/cells9010132

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Actomyosin activity increases SUN2 mobility and the interaction between SUN2 and lamin A. (A) Representative prebleach, bleach, and recovery images of control, Y27632- and blebbistatin-treated GFP-SUN2 expressing VSMCs grown on 12 kPa hydrogels (arrow marks the bleached region). Graphs show (B) fluorescence recovery profile, (C) mobile fraction, and (D) T1/2 of GFP-SUN2 recovery in control (n = 21), Y27632 (n = 18)-, and blebbistatin (n = 22)-treated VSMCs (* p < 0.05 and ** p < 0.01). (E) Representative prebleach and post-bleach images of control, Y27632-, and blebbistatin-treated VSMCs expressing GFP-SUN2 and RFP-lamin A grown on 12 kPa hydrogels. (F) Graph shows GFP-SUN2/RFP-lamin A and GFP/RFP-lamin A FRET efficiency of control (n = 22), Y27632 (n = 19)-, and blebbistatin (n = 24)-treated VSMCs (* p < 0.05 and ** p < 0.01).