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Optimization of the eukaryotic initiation factor 6 (eIF6)–60S binding assay. (A) Schematic drawing of AlphaScreen technology. (B) Optimization of the 60S-bio signal against a fixed concentration of eIF6. (C) eIF6 Kd value measurement. (D) Titration of non-biotinylated 60S displacement of eIF6 from biotynilated 60S. The results show that the assay is suitable for detecting disruptors of the 60S–eIF6 binding.
