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E2F1 and E2F4 were downregulated by OTA exposure. (A) E2F1 and E2F4 were significantly downregulated following 100 nM OTA exposure according to RNA-sequencing data in both cell lines, while E2F7 was found to be upregulated only in HEK293-T cells. Due to the consistent results between the two cell lines, the expression of E2F1 and E2F4 only was verified by RT-qPCR (B) and Western blot (C) (mean ± SD, * p < 0.05, N = 3).
