Figure 3.

miR-340-3p and miR-132-5p induce TNF release from murine microglia and human THP-1 cell-derived monocytes. Murine microglia from C57BL/6 mice (a) and human THP-1-derived monocytes (b) were incubated with 10 µg/mL of the indicated miRNAs for 24 h. Loxoribine (TLR7), resiquimode (R848; TLR7/8), and lipopolysaccharide (LPS) (TLR4) served as positive controls, while unstimulated condition and LyoVec were used as a negative control. Mutant oligoribonucleotide (ctrl oligo, 10 µg/mL) was used as a further negative control. TNF amounts in supernatants were determined via ELISA and are shown as fold change normalized to unstimulated control. Significances were obtained by Student’s t-Test. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; * compared to unstimulated control). Data of n = 3 are represented as mean + s.e.m.