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. Author manuscript; available in PMC: 2020 Feb 13.
Published in final edited form as: Biochemistry. 2019 Apr 17;58(17):2260–2268. doi: 10.1021/acs.biochem.8b00939

Figure 2.

Figure 2.

Inhibition of N5-CAIR synthetase by isatin in the ATP-NADH coupled assay. The ATP-NADH coupled N5-CAIR synthetase reaction was initiated with AIR (25 μM) and bromoisatin (100 μM) at time zero, and the conversion of NADH to NAD+ was measured at 340 nm. Seventy nanograms of the A. clavatus enzyme (A) or 110 ng of the E. coli enzyme (B) was used in the assay. (C) 4-Bromo-, 5-bromo-, and 7-bromoisatin from left to right, respectively. Legend: no isatin (●), 4-bromoisatin (■), 7-bromoisatin (▲), and 5-bromoisatin (◆). The Y-axes have different scales, from 0.85 to 1.00 for panel A and from 0.70 to 1.00 for panel B. The lines in all graphs are the best-fit lines as executed by KaleidaGraph. The experiments generated continuous data; however, only a limited number of data points for each curve are shown for the sake of clarity.