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. 2019 Dec 24;5(12):e03057. doi: 10.1016/j.heliyon.2019.e03057

Figure 3.

Figure 3

Damaged chromatin loading of PolQ and MRE11 are more effective under MMR deficient background compared to those of WT cells. (A,B) WT and Msh2−/− MEFs were treated with HU as shown in the workflow, and then IP (A) and chromatin fractionation (B) was performed. IP was performed with cells at 1 h after release from HU using an anti-Rad51 or -Flag antibody. Source images of blots are shown in Supplementary Fig. 4. Their reciprocal associations were detected by immunoblotting (IB). H3 was detected as a loading control (B). Source images of blots are shown in Supplementary Fig. 5.