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. 2020 Jan 12;10(5):2243–2259. doi: 10.7150/thno.35861

Figure 2.

Figure 2

ZNF471 suppresses ESCC cell proliferation and induces G0/G1 arrest and apoptosis. (A, B) Ectopic expression of ZNF471 in ESCC cell lines was measured by RT-PCR and western blotting. (C) Colony formation of vector- and of ZNF471-transfected KYSE150 and KYSE410 cells (left panel). Rates are shown as mean ± SD (right panel) from three independent experiments. (D) Cell viabilities evaluated at 24, 48 and 72 h after transfection with ZNF471 in KYSE150 and KYSE410 cells. (E) Cell cycle distribution measured in vector- and ZNF471-stably transfected KYSE150 and KYSE410 cells by flow cytometry. Representative distribution plots and histograms of alterations are shown. (F) Percentages of apoptotic cells in KYSE150 and KYSE410 cells with ZNF471 ectopic expression were evaluated. Cell apoptosis alterations were revealed by histograms. (G) Expression of Cyclin D1, p21, p27, cleaved -caspase 8, -caspase 3 and -PARP measured by western blotting in ZNF471-transfected ESCC cells. Student's test was used. Data are presented as the mean ± SD. *p<0.05, ***p<0.001.