Effect of GJ and CDDP on cell viability of U87MG and U373MG cells. MTT assays were performed in (a) GJ-treated and (b) CDDP-treated U87MG cells. (c) Microscopical observation of cell morphology was performed in GJ- and CDDP-treated U87MG cells. (d) Western blot assays on cleaved caspase-3, cleaved caspase-9, and cleaved poly (ADP-ribose) polymerase (PARP) were performed in GJ- and CDDP-treated U87MG cells. MTT assays were performed in (e) GJ-treated and (f) CDDP-treated U373MG cells. (g) Microscopical observation of cell morphology was performed in GJ- and CDDP-treated U373MG cells. Results are displayed as mean ± S.E. of three or more separate experiments. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as endogenous control. * p < 0.05 vs. untreated cells, ** p < 0.01 vs. untreated cells, *** p < 0.005 vs. untreated cells. GJ, Gardenia jasminoides; CDDP, cisplatin.