Table 2.

Comparison of IHC and the LN34 Taqman Real-Time RT-PCR assay using multiple sections of brain tissues of A18-2173.

IHC RABV Distribution/Intensity	Real Time RT-PCR
	LN34 Ct	β-Actin * Ct	Result
2+/4+	30.7	24.5	Positive
2+/4+	26.1	27.6	Positive
2+/4+	26.9	22.4	Positive
<2+/4+	34.5	24.1	Positive
<2+/4+	35	24.8	Positive
<2+/4+	32.5	26	Positive
Not tested white matter	30.7	24	Positive
Not tested gray matter	Undetected	25.5	Negative

* qPCR assay measures host RNA level.