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. 2020 Jan 20;12(1):265. doi: 10.3390/nu12010265

Table 2.

Primer sequences of detected genes.

Gene Primer Sequences Tm Sequence Number Product PCR (bp)
PPARγ Forward TTTTCAAGGGTGCCAGTTTCGATCC 61 NM_001127330.1 198
Reverse AATCCTTGGCCCTCTGAGAT 58
ACC Forward GGCCAGTGCTATGCTGAGAT 59 NM_133360.2 108
Reverse AGGGTCAAGTGCTGCTCCA 59
FAS Forward GCTGCGGAAACTTCAGGAAAT 57 NM_007988.3 84
Reverse AGAGACGTGTCACTCCTGGACTT 61
SREBP-1c Forward CACAGCGGTTTTGAACGACA 58 NM_011480.3 147
Reverse CTCTCAGGAGAGTTGGCACC 58
HSL Forward CTGGAACTAAGTGGACGCAAG 56 NM_001039507 91
Reverse CAGACACACTCCTGCGCATAGAC 60
C/EBP-α Forward TCGGTGCGTCTAAGATGAGG 57 NM_001287523 179
Reverse TCAAGGCACATTTTTGCTCC 55
SCD1 Forward AGAGAACTGGAGACGGGAGT 57 NM_009127 130
Reverse AACACCCCGATAGCAATATCCA 57
PPARα Forward CAAGGCCTCAGGGTACCACT 59 NM_001113418.1 111
Reverse TTGCAGCTCCGATCACACTT 58
CPT-1 Forward CTCAGTGGGAGCGACTCTTCA 59 NM_013495.2 105
Reverse GGCCTCTGTGGTACACGACAA 60
ACOX Forward GCCTTTGTTGTCCCTATCCG 58 NM_001271898.1 189
Reverse TACATACGTGCCGTCAGGC 58
β-actin Forward CAGGCATTGCTGACAGGATG 58 NM_007393 156
Reverse TGCTGATCCACATCTGCTGG 58