Table 2.
Gene | Primer Sequences | Tm | Sequence Number | Product PCR (bp) | |
---|---|---|---|---|---|
PPARγ | Forward | TTTTCAAGGGTGCCAGTTTCGATCC | 61 | NM_001127330.1 | 198 |
Reverse | AATCCTTGGCCCTCTGAGAT | 58 | |||
ACC | Forward | GGCCAGTGCTATGCTGAGAT | 59 | NM_133360.2 | 108 |
Reverse | AGGGTCAAGTGCTGCTCCA | 59 | |||
FAS | Forward | GCTGCGGAAACTTCAGGAAAT | 57 | NM_007988.3 | 84 |
Reverse | AGAGACGTGTCACTCCTGGACTT | 61 | |||
SREBP-1c | Forward | CACAGCGGTTTTGAACGACA | 58 | NM_011480.3 | 147 |
Reverse | CTCTCAGGAGAGTTGGCACC | 58 | |||
HSL | Forward | CTGGAACTAAGTGGACGCAAG | 56 | NM_001039507 | 91 |
Reverse | CAGACACACTCCTGCGCATAGAC | 60 | |||
C/EBP-α | Forward | TCGGTGCGTCTAAGATGAGG | 57 | NM_001287523 | 179 |
Reverse | TCAAGGCACATTTTTGCTCC | 55 | |||
SCD1 | Forward | AGAGAACTGGAGACGGGAGT | 57 | NM_009127 | 130 |
Reverse | AACACCCCGATAGCAATATCCA | 57 | |||
PPARα | Forward | CAAGGCCTCAGGGTACCACT | 59 | NM_001113418.1 | 111 |
Reverse | TTGCAGCTCCGATCACACTT | 58 | |||
CPT-1 | Forward | CTCAGTGGGAGCGACTCTTCA | 59 | NM_013495.2 | 105 |
Reverse | GGCCTCTGTGGTACACGACAA | 60 | |||
ACOX | Forward | GCCTTTGTTGTCCCTATCCG | 58 | NM_001271898.1 | 189 |
Reverse | TACATACGTGCCGTCAGGC | 58 | |||
β-actin | Forward | CAGGCATTGCTGACAGGATG | 58 | NM_007393 | 156 |
Reverse | TGCTGATCCACATCTGCTGG | 58 |