Figure 4.

Changes in cell membrane fluidity expressed as values of generalized polarization (GP) for Laurdan probe after incubation of cells with T. pratense at IC₀ (A). The effect of T. pratense extracts on mitochondrial membrane potential was determined with JC-1 probe. As a positive control for depolarization, carbonyl cyanide m-chlorophenyl hydrazine (CCCP) (50 μM) was used (B). control cells were not exposed to any compound but the vehicle; values are means ± standard deviations from at least three independent experiments; statistical significance was calculated versus control cells (untreated); * p ≤ 0.05, *** p ≤ 0.001.